Name: inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models
Uploaded: 2014-05-14T13:00:00
Description: Watch this Scientific Journal Video about Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models at JoVE.com

作者感谢克里斯蒂安Pahrmann的技术援助。 SS收到的资金从德意志研究联合会（DFG）（SCHR992/3-2和SCHR992/4-2）。

所有的动物工作应按照有关动物护理指引进行。获得批准的机构动物工作开始前协议。 1，肌内膜增生模型（一） 对于血管内膜增生模型，购买C57BL/6J（股票编号000664，C57BL/6J）小鼠在8周体重大约25克的年龄。房子老鼠常规条件下这些实验;喂小鼠标准鼠标饲料，并提供水随意 。 <ol><li> 鼠标的准备：介绍鼠标采用感应腔麻醉状态...

<ol>
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R., Yu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simplified+murine+intimal+hyperplasia+model+founded+on+a+focal+carotid+stenosis.">A simplified murine intimal hyperplasia model founded on a focal carotid stenosis.</a> Am J Pathol. 182, 277-287 (2013).</li><li>Kumar, A., Lindner, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remodeling+with+neointima+formation+in+the+mouse+carotid+artery+after+cessation+of+blood+flow.">Remodeling with neointima formation in the mouse carotid artery after cessation of blood flow.</a> Arterioscler Thromb Vasc Biol. 17, 2238-2244 (1997).</li><li>Hua, X., Deuse, T., Michelakis, E. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+internal+mammary+artery+(IMA)+transplantation+and+stenting:+a+human+model+to+study+the+development+of+in-stent+restenosis.">Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis.</a> J Vis Exp. , 3663 (2012).</li><li>Daugherty, A., Whitman, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+atherosclerosis+in+mice.">Quantification of atherosclerosis in mice.</a> Methods Mol Biol. , (2003).</li><li>Nakashima, Y., Plump, A. S., Raines, E. W., Breslow, J. 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虽然这两种疾病导致在患者类似的症状，斑块发展的基本机制，因此，治疗方法有很大的不同2。在不同形式的动脉狭窄的病人的临床表现以及斑块发展的时间表取决于底层机制。 取决于在患者中的临床发现，样品的分析方法的不同，必须执行5,15。有必要分析适应于这种疾病的特征的研究结果，是被模仿由所选择的模型14。反之亦然，这取决于疾病正?...

在工业化国家，缺血性心脏疾病的死亡率仍1的主要原因。起因于冠状动脉狭窄是多方面的，包括肌内膜增生和动脉粥样硬化，血管再通与剩余的最常见的治疗策略2。研究模型内膜增生和动脉粥样硬化的机制途径明确区分是必不可少的探讨动脉斑块发展的病理生物学和病理生理过程。在这段视频中用于研究肌内膜增生或动脉粥样硬化无论是发展的两个不同的小鼠模型进行了介绍。 肌内膜增生是假设，形成通过最初描述为动脉粥样硬化3“响应到伤害”的范式。内皮细胞层的机械破坏导致一个强烈的重塑通过旁分泌作用增强。有几种二fferent动物模型通常用于研究肌内膜增生。有些团体使用的金属器件在大鼠4的升主动脉。用气囊导管进行的主动脉剥脱模型也常用于实验室大鼠5,6。在实验室老鼠7执行的血管内膜增生模型实现了颈动脉结扎诱导肌内膜病变8。在我们的实验室中，腹主动脉剥脱模型-研究肌内膜增生的发展-以及一个人性化的支架内再狭窄模型9常用。这部影片强调，选择合适的实验动物模型是动脉狭窄的机械或病理生理的研究是至关重要的。 肌内膜增生模型必须从动脉粥样硬化模型的区别。对于后者，载脂蛋白E缺陷（ApoE基因 - / - ）小鼠与西方饮食组合常用于诱导atheroscl色情病变10,11,12,13。对于这两种类型的小鼠肌内膜疾病的诱导例子如下所示，随着不同的模型适应选择，分析血管狭窄14。

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		<col />
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		<tr height="17">
			<td height="17" style="height:17px;width:156px;">Name</td>
			<td style="width:183px;">Company</td>
			<td style="width:139px;">Catalog number</td>
			<td style="width:140px;">Comments</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Thyoglycollate Broth 3%</td>
			<td>Fluka</td>
			<td align="right">70157</td>
			<td>powder</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">PFA 4%</td>
			<td>Electron Microscopy Sciences</td>
			<td>#157135S</td>
			<td align="right">20%</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Sudan III staining solution</td>
			<td>Sigma Aldrich</td>
			<td>S4131</td>
			<td>powder</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">mouse C57BL/6J</td>
			<td>Jackson Laboratories</td>
			<td>&#160;Stock # 0006664</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">mouse ApoE-/-</td>
			<td>Jackson Laboratories</td>
			<td>&#160;Stock #002052</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Western Diet</td>
			<td>Harlan Laboratories</td>
			<td>TD.88137</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">hair clipper</td>
			<td>WAHL</td>
			<td>8786-451A ARCO SE</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Forene</td>
			<td>Abbott</td>
			<td></td>
			<td>Isoflurane</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">microsurgical clamp</td>
			<td>Fine Science Tools</td>
			<td>18055-04</td>
			<td>Micro-Serrefine - 4mm</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">clamp applicator</td>
			<td>Fine Science Tools</td>
			<td>18056-14</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">catheter</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">10-0 prolene suture</td>
			<td>Ethicon</td>
			<td>788G</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">6-0 prolene suture</td>
			<td>Ethicon</td>
			<td>8709H</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">5-0 prolene suture</td>
			<td>Ethicon</td>
			<td>EH7229H</td>
			<td></td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Rimadyl</td>
			<td>Pfizer&#160;</td>
			<td></td>
			<td>Carprofen</td>
		</tr>
		<tr height="17">
			<td height="17" style="height:17px;">Metacam 1.5mg/ml</td>
			<td>Boehringer Ingelheim</td>
			<td></td>
			<td>Metamizol</td>
		</tr>
	</tbody>
</table>

inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models

已经建立了各种体内实验室啮齿动物模型中对动脉狭窄的诱导以模仿包括动脉斑块的形成和狭窄，如在缺血性心脏疾病观察例如疾病。两个高度可重复的小鼠模型 - 既导致动脉狭窄，但每个底层不同的途径发展 - 在这里介绍。该模型代表动脉狭窄的两种最常见的原因;即会显示一个鼠标型号为每个肌内膜增生，动脉粥样硬化。为了诱导肌内膜增生，进行腹主动脉球囊导管损伤。对于粥样硬化斑块的发展，ApoE基因 - / - 与西方脂肪的饮食组合的小鼠模型被使用。对结果的衡量和评估不同的模式适应选项将在本手稿命名和描述。这两款车型的引进和比较提供了信息离子为科学家选择在按照要求科学问题的相应动脉狭窄模型。

图1显示了不同的分析选项，并为这两个模型中引起的疾病状态。对于内膜增生模型，示出了在三色染色的代表横截面的分析。从这些横截面，如I / M比，最大斑块厚度，最大内膜厚度在管腔内，以百分比管腔闭塞，以及斑块面积的结果可以被测量和计算。 为了评估动脉粥样硬化模型中的结果，可以使用所有上面提到的方法。此外，定量斑块面积在腹主动脉，斑?...

Watch this Scientific Journal Video about Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models at JoVE.com

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

诱导肌内膜增生对战动脉粥样硬化小鼠：两种有效的模型介绍

该视频显示两个模型内膜斑块的发展在小鼠动脉，并强调在肌内膜增生和动脉粥样硬化的差异。

Various in vivo laboratory rodent models for the induction of artery stenosis have been established to mimic diseases that include arterial plaque ...

Research

JoVE Journal

Medicine

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

植入小鼠触发剪应力致动脉粥样硬化的颈袖

It is widely accepted that alterations in vascular shear stress trigger the expression of inflammatory genes in endothelial cells and thereby induce ...

科研

医学

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

急性脑外伤小鼠其次是纵向双光子成像

Although acute brain trauma often results from head damage in different accidents and affects a substantial fraction of the population, there is no ...

Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice

在诱导小鼠心肌梗死的微创手术过程

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last ...

Step By Step: Microsurgical training method combining two nonliving animal models

一步一步：显微外科培训方法相结合的两个无生命的动物模型

The learning of microsurgical techniques and the maintenance of microsurgical skills have been traditionally based on the use of living animals, mainly ...

Surgical Models of Gastroesophageal Reflux with Mice

胃食管反流与小鼠手术模型

Multiple surgical procedures have been reported to induce gastroesophageal reflux in animals. Herein, we report three surgical models with mice aiming to ...

An Improved Method for Rapid Intubation of the Trachea in Mice

在小鼠气管插管快速的改进方法

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation

Glaucoma-inducing Procedure in an In Vivo Rat Model and Whole-mount Retina Preparation

青光眼诱导在程序<em&gt;在体内</em&gt;大鼠模型和全安装视网膜准备

Glaucoma is a disease of the central nervous system affecting retinal ganglion cells (RGCs). RGC axons making up the optic nerve carry visual input to the ...

Induction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model

小鼠加速动脉硬化的诱导："电线损伤"模型

Atherosclerosis is a proliferative fibro-inflammatory disease developing in the arterial wall, inducing a deficient blood flow or a lack of blood flow. ...

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

球囊损伤诱发小鼠腹主动脉 Myointimal 增生的研究

The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine ...