Name: inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models
Uploaded: 2014-05-14T13:00:00
Description: Watch this Scientific Journal Video about Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models at JoVE.com

Forfatterne takker Christiane Pahrmann for teknisk assistanse. SS fått støtte fra Deutsche Forschungsgemeinschaft (DFG) (SCHR992/3-2 og SCHR992/4-2).

Alle dyr arbeid skal utføres i henhold til gjeldende retningslinjer dyr omsorg. Skaff institusjonell godkjenning for dyr arbeid før begynnelsen protokollen. En. Myointimal Hyperplasi Modell (A) For intimal hyperplasia modell, kjøpe C57BL/6J (Stock nummer 000664, C57BL/6J) mus i en alder av 8 uker som veier ca 25 g. Hus mus for disse eksperimentene henhold konvensjonelle forhold; mate mus standard mus chow og gi vann ad libitum. <ol>...

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	<li>Manuel, D. G., Leung, M., Nguyen, K., Tanuseputro, P., Johansen, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Burden+of+cardiovascular+disease+in+Canada.">Burden of cardiovascular disease in Canada.</a> Can J Cardiol. 19, 997-1004 (2003).</li><li>ER, O. B., Ma, X., Simard, T., Pourdjabbar, A., Hibbert, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogenesis+of+neointima+formation+following+vascular+injury.">Pathogenesis of neointima formation following vascular injury.</a> Cardiovasc Hematol Disord Drug Targets. 11, 30-39 (2011).</li><li>Ross, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pathogenesis+of+atherosclerosis:+a+perspective+for+the+1990s.">The pathogenesis of atherosclerosis: a perspective for the 1990s.</a> Nature. 362, 801-809 (1993).</li><li>Meng, X., Zhang, F., Valji, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+guidance+for+the+creation+of+a+small+animal+model+of+aortic+injury.">Ultrasound guidance for the creation of a small animal model of aortic injury.</a> J Vasc Interv Radiol. 22, 1193-1197 (2011).</li><li>Deuse, T., Koyanagi, T., Erben, R. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustained+inhibition+of+epsilon+protein+kinase+C+inhibits+vascular+restenosis+after+balloon+injury+and+stenting.">Sustained inhibition of epsilon protein kinase C inhibits vascular restenosis after balloon injury and stenting.</a> Circulation. 122, 170-178 (2010).</li><li>Schrepfer, S., Deuse, T., Sultan, K. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+restenosis+development+after+mechanical+injury:+a+new+field+of+application+for+malononitrilamides.">Inhibition of restenosis development after mechanical injury: a new field of application for malononitrilamides.</a> Cardiology. 108, 128-137 (2007).</li><li>Tao, M., Mauro, C. R., Yu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simplified+murine+intimal+hyperplasia+model+founded+on+a+focal+carotid+stenosis.">A simplified murine intimal hyperplasia model founded on a focal carotid stenosis.</a> Am J Pathol. 182, 277-287 (2013).</li><li>Kumar, A., Lindner, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remodeling+with+neointima+formation+in+the+mouse+carotid+artery+after+cessation+of+blood+flow.">Remodeling with neointima formation in the mouse carotid artery after cessation of blood flow.</a> Arterioscler Thromb Vasc Biol. 17, 2238-2244 (1997).</li><li>Hua, X., Deuse, T., Michelakis, E. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+internal+mammary+artery+(IMA)+transplantation+and+stenting:+a+human+model+to+study+the+development+of+in-stent+restenosis.">Human internal mammary artery (IMA) transplantation and stenting: a human model to study the development of in-stent restenosis.</a> J Vis Exp. , 3663 (2012).</li><li>Daugherty, A., Whitman, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+atherosclerosis+in+mice.">Quantification of atherosclerosis in mice.</a> Methods Mol Biol. , (2003).</li><li>Nakashima, Y., Plump, A. S., Raines, E. W., Breslow, J. L., Ross, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ApoE-deficient+mice+develop+lesions+of+all+phases+of+atherosclerosis+throughout+the+arterial+tree.">ApoE-deficient mice develop lesions of all phases of atherosclerosis throughout the arterial tree.</a> Arterioscler Thromb. 14, 133-1340 (1994).</li><li>Zhang, Y., Li, L., You, J., Cao, J., Fu, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+7-difluoromethyl-5,+4'-dimethoxygenistein+on+aorta+atherosclerosis+in+hyperlipidemia+ApoE(-/-)+mice+induced+by+a+cholesterol-rich+diet.">Effect of 7-difluoromethyl-5, 4'-dimethoxygenistein on aorta atherosclerosis in hyperlipidemia ApoE(-/-) mice induced by a cholesterol-rich diet.</a> Drug Des Devel Ther. 7, 233-242 (2013).</li><li>Rudolph, T. K., Rudolph, V., Edreira, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nitro-fatty+acids+reduce+atherosclerosis+in+apolipoprotein+E-deficient+mice.">Nitro-fatty acids reduce atherosclerosis in apolipoprotein E-deficient mice.</a> Arterioscler Thromb Vasc Biol. 30, 938-9345 (2010).</li><li>Rondelet, B., Kerbaul, F., Motte, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bosentan+for+the+prevention+of+overcirculation-induced+experimental+pulmonary+arterial+hypertension.">Bosentan for the prevention of overcirculation-induced experimental pulmonary arterial hypertension.</a> Circulation. 107, 1329-1335 (2003).</li><li>Zeibig, S., Li, Z., Wagner, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+the+oxLDL+binding+protein+Fc-CD68+on+plaque+extension+and+vulnerability+in+atherosclerosis.">Effect of the oxLDL binding protein Fc-CD68 on plaque extension and vulnerability in atherosclerosis.</a> Circ Res. 108, 695-703 (2011).</li><li>Painter, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myointimal+hyperplasia:+pathogenesis+and+implications.+2.+Animal+injury+models+and+mechanical+factors.">Myointimal hyperplasia: pathogenesis and implications. 2. Animal injury models and mechanical factors.</a> Artificial organs. 15, 103-118 (1991).</li><li>Nitschke, Y., Weissen-Plenz, G., Terkeltaub, R., Rutsch, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Npp1+promotes+atherosclerosis+in+ApoE+knockout+mice.">Npp1 promotes atherosclerosis in ApoE knockout mice.</a> Journal of cellular and molecular. 15, 2273-2283 (2011).</li></ol>

Selv om begge sykdommene gi lignende symptomer hos pasienten, de underliggende mekanismene for plakk utvikling og dermed de behandlingsmetoder er svært forskjellige to. I forskjellige former av arteriell stenose de kliniske funn i pasienter så vel som den tidslengde for utvikling av plaque, avhenger av den underliggende mekanisme. Avhengig av de kliniske funn hos pasienten, må ulike fremgangsmåter for analyse av prøver utføres 5,15. Det er nødvendig å tilpasse a...

I industrialiserte land iskemisk hjertesykdom er fortsatt en ledende dødsårsak en. Årsakene til koronar stenose er mangfoldige og omfatter myointimal hyperplasia samt aterosklerose, med revaskularisering rester den vanligste behandlingsstrategi to. Forskningsmodeller som klart skiller mellom de mekanistiske veier av intimal hyperplasi og aterosklerose er viktig å undersøke pathobiological og patofysiologiske prosesser i arteriell plakk utvikling. I denne video to forskjellige musemodeller som brukes til å studere enten utvikling av myointimal hyperplasia, eller aterosklerose er innført. Myointimal hyperplasia er postulert å danne via &quot;respons-til-skade&quot; paradigme opprinnelig beskrevet for aterosklerose tre. Den mekaniske forstyrrelse av endoteliale sjikt fører til en intens ombygging forsterket av parakrine effekter. Det er flere different dyremodeller som vanligvis brukes til å studere myointimal hyperplasia. Noen grupper bruker metall enheter i stigende aorta av rotter fire. Den aorta denudation modellen utføres med et ballongkateter blir også ofte brukt i laboratorierotter 5,6. Den intimal hyperplasia modell utført i laboratoriet mus 7 implementerer ligation av halspulsåren og induserer myointimal lesjoner åtte. I vårt laboratorium, abdominal aorta-modell denudation - for å studere utviklingen av myointimal hyperplasia - så vel som et humanisert stent restenosis modell 9 blir ofte brukt. Denne videoen understreker at det å velge riktig eksperimentell dyremodell er avgjørende for mekanistiske eller pathophysiologic studier av arteriell stenose. Myointimal hyperplasi modeller må skilles fra aterosklerose modeller. For sistnevnte, apolipoprotein E-mangel (ApoE - / -) er mus i kombinasjon med vestlig kosthold som vanligvis brukes til å indusere atherosclerotiske lesjoner 10,11,12,13. Eksempler for induksjon av begge disse typer av myointimal sykdom hos mus er vist her, sammen med forskjellige modell innrettet muligheter for å analysere fartøy stenose 14..

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			<td>powder</td>
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			<td>Electron Microscopy Sciences</td>
			<td>#157135S</td>
			<td align="right">20%</td>
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			<td height="17" style="height:17px;">Sudan III staining solution</td>
			<td>Sigma Aldrich</td>
			<td>S4131</td>
			<td>powder</td>
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			<td height="17" style="height:17px;">mouse C57BL/6J</td>
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			<td>&#160;Stock # 0006664</td>
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			<td height="17" style="height:17px;">Forene</td>
			<td>Abbott</td>
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			<td height="17" style="height:17px;">microsurgical clamp</td>
			<td>Fine Science Tools</td>
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			<td>Fine Science Tools</td>
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			<td height="17" style="height:17px;">catheter</td>
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			<td height="17" style="height:17px;">6-0 prolene suture</td>
			<td>Ethicon</td>
			<td>8709H</td>
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			<td height="17" style="height:17px;">5-0 prolene suture</td>
			<td>Ethicon</td>
			<td>EH7229H</td>
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			<td height="17" style="height:17px;">Rimadyl</td>
			<td>Pfizer&#160;</td>
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			<td>Carprofen</td>
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			<td height="17" style="height:17px;">Metacam 1.5mg/ml</td>
			<td>Boehringer Ingelheim</td>
			<td></td>
			<td>Metamizol</td>
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inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models

Forskjellige in vivo-gnagerlaboratoriemodeller for induksjon av arteriestenose er blitt etablert for å etterligne sykdommer som inkluderer arteriell plaque dannelse og stenose, som observert for eksempel ischemisk hjertesykdom. To svært reproduserbare musemodeller - både som resulterer i arteriestenose men hver underliggende en annen vei for utvikling - er innført her. Modellene representerer de to vanligste årsakene til nyrearteriestenose; nemlig en musemodell for hver myointimal hyperplasia, og aterosklerose er vist. Å indusere myointimal hyperplasi, er et ballongkateter skade av abdominal aorta utført. For utviklingen av aterosklerotisk plakk, til ApoE - / - er musemodellen i kombinasjon med western fet diett anvendes. Ulike modelltilpassede alternativer for måling og evaluering av resultatene er navngitt og beskrevet i dette manuskriptet. Innføringen og sammenligning av disse to modellene gir information for forskere å velge riktig arteriestenose modell i henhold til den vitenskapelige spørsmål spurt.

Figur 1 viser ulike analysemuligheter og den induserte sykdomstilstand for begge modellene. For intimal hyperplasia modell, er analysen av representative tverrsnitt i Trikrom farging vist. Fra disse tverrsnitt, kan resultatene som I / M-forhold, maksimal plakktykkelse, maksimal intimal tykkelse i lumen, luminal utslettelse i prosent, samt plakkområdet måles og beregnes. For å vurdere resultatet av aterosklerose modell, kan alle metoder som er nevnt ovenfor brukes. I tille...

Watch this Scientific Journal Video about Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models at JoVE.com

Inducing Myointimal Hyperplasia Versus Atherosclerosis in Mice: An Introduction of Two Valid Models

Inducing Myointimal Hyperplasi Versus Åreforkalkning i Mus: En introduksjon av to gyldige modeller

Denne videoen viser to modeller av intimal plakk utvikling i murine arterier og understreker forskjellene i myointimal hyperplasi og åreforkalkning.

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The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine ...