Name: quantification of global diastolic function by kinematic modeling-based analysis of transmitral flow via the parametrized diastolic filling formalism
Uploaded: 2014-09-01T14:00:00
Description: Watch this Scientific Journal Video about Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism at JoVE.

这项工作是由阿兰。和伊迪丝L.沃尔夫慈善基金，圣路易斯和巴恩斯犹太医院基金会赞助。属Shmuylovich和E戈什由美国心脏协会的中心地带加盟博士前奖学金的发放进行了部分支持。朱南接受部分支持来自华盛顿大学的康普顿奖学金项目和艺术学院与科学“暑期本科生研究奖。南Mossahebi获得了部分支持物理系。

获取超声图像，并对其进行分析，以获得PDF格式参数的过程详述如下。虽然心导管被提及在下面的受试者选择部分，所描述的方法只适用于超声心动图的部分。在导管部分的描述被列入为基础的模型预测的独立验证，是无关的电子波通过 PDF形式主义的分析。此前数据采集，所有科目提供签署知情同意参与这项研究符合机构审查委员会（人类研究保护办公室）在华盛顿大学医学院。 <p clas...

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按照我们的方法学重点，这有利于获得准确和有意义的结果的方法的关键环节突出显示。 超声心动图美国超声心动图学会（ASE）有胸研究16的性能准则。期间的回声检查，有众多的影响图像质量的因素。这超出了超声医师的控制因素包括：热像仪的技术能力被利用，心脏率，患者的体型，在位置的个体差异，解剖结构的方向和“回声窗​​”的质...

由卡茨1于1930年开创性的研究表明，哺乳动物左心室由是机械抽气泵，从那时起一直致力于解开舒张的运作多的努力开始灌装。多年以来，侵入性方法，可供舒张功能（DF）2-16临床或科研评价的唯一选择。在20世纪70年代，但是，技术进步和发展，超声心动图终于给了心脏病学家和生理学家实用的工具，东风无创性的表征。 如果没有一个统一的因果理论或范式关于如何当它填补了心脏舒张的作品，研究人员提出了一种基于临床特征的关系很多phenomenologic指标。的曲线，迅速上升，并在早，迅速填充落入二尖瓣血流速度轮廓的形状，例如，被近似为三角形和舒张复nction索引是从几何特征限定了三角形的（高度，宽度，面积等 ）。在超声心动图技术的进步已经允许组织运动，应变和应变率填充到被测量，例如在，并带来了现象学指数的新作物各技术进步与临床特征相关。不过，指数仍然相关，而不是因果关系，很多指标都是相同的底层生理不同的措施。这并不奇怪，因此，东风目前使用的临床指标有限的特异性和灵敏度。 为了克服这些限制的参数化舒张功能（PDF格式）形式主义，因果运动，左室充盈是由动机，并采用舒张的抽吸泵生理学集总参数模型的开发和验证17。该模型舒张功能（如表现在曲线形状的静脉流轮廓）按照阻尼谐波振荡运动的规则。该方程阻尼谐波振荡运动是基于牛顿第二定律和可写，每单位质量，如： <img alt="式（1）" fo:content-width="1in" src="/files/ftp_upload/51471/51471eq1.jpg" /> 式（1） 这种线性2阶差分方程有三个参数：K -腔僵硬，C -粘弹性/松弛，和×○ -振荡器的初始位移/预紧力。该模型预测，不同临床观察舒张图案变化在这三个模型参数的数值的结果。基于PDF形式主义和经典力学，电子波可以归类为欠阻尼或过阻尼运动的制度来确定。许多研究<suP&gt; 17-21已经证实，临床上记录的E波的轮廓和PDF模型预测的轮廓展现高超的协议，并已阐明三个PDF格式的血流动力学/生理类似物参数21。这个过程从临床记录的E波的数据中提取模型参数是在下面的方法的详细。 与东风在目前临床使用的典型指标，PDF格式模型的三个参数是基于因果关系。如下面的方法所讨论的，舒张生理学的额外的索引可以从这些基本参数和从应用程序中的PDF形式主义的舒张其他方面比静脉流。在这项工作中，静脉流和生理关系的基于PDF的分析，可以从PDF方法绘制的方法，其参数与导出指标进行说明。另外，它表明，从它们衍生的PDF参数或索引可以逗从负载的外部效应内在相距室性可以提供关联到传统的侵入性定义的参数和正常和病理组之间可以分化。

<table border="0" cellpadding="0" cellspacing="0" width="661">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Equipment/ Software</td>
			<td style="width:159px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Philips iE33</td>
			<td style="width:159px;">Philips (Andover, MA.)</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">LabVIEW 6.0</td>
			<td style="width:159px;">National Instruments</td>
			<td style="width:119px;"></td>
			<td>Version 6.0.2</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">MATLAB</td>
			<td style="width:159px;">MathWorks</td>
			<td style="width:119px;"></td>
			<td>Version R2010b</td>
		</tr>
	</tbody>
</table>

quantification of global diastolic function by kinematic modeling-based analysis of transmitral flow via the parametrized diastolic filling formalism

定量心脏功能的评估仍然是生理学家和临床医生的一个挑战。尽管历史上有创方法都包含唯一的手段可用，无创成像方式的发展（超声心动图，MRI，CT），具有高时空分辨率为定量舒张功能评估的新窗口。超声心动图是同意的标准舒张功能的评估，但目前临床使用的索引仅仅是利用所选室尺寸（M模式）或血液/组织运动（多普勒）波形的功能，而结合了运动本身的生理因果决定因素。所有的左心室（LV）的启动按作为机械吸泵灌装承认允许根据适用于所有室内运动定律全球舒张功能进行评估。什么从另一个区分各个心脏的运动是GOV方程的参数ERNS灌装。因此，参数化舒张期充盈的发展（PDF格式）形式主义表明，临床上观察到的早期二尖瓣流量的整个范围（多普勒E波）模式是非常好的契合了阻尼振荡运动的规律。这允许按照因果机制（反冲启动吸）能产生3（数字）独特的集总参数，其生理类似物室刚度（K），粘弹性/休闲（c）和负载（X个人电子波的分析O）。静脉流（多普勒电子波）的记录是在临床心脏病学的标准做法，因此，超声心动图的记录方法只简要回顾。我们的重点是确定从日常记录的电子波数据的PDF参数。作为突出的结果表明，一旦PDF参数已经从负载的适当数量而获得不同的E-波的不变拟tigator是免费使用的参数或者参数的构造指标（如储存能量半KX O 2，最大的AV压力梯度KX O，舒张功能的负载独立指数等 ），并选择生理或病理生理的角度进行量化。

多普勒波形代表了四种不同类型的填充图案的使用上文详述的方法（正常，假性，延迟松弛，缩窄限制性的）示于图2的图2A中示出的正常模式，其中，其本身是从假性难以区别图案。 图2B显示延迟的放松和图2C显示了严重的舒张功能障碍相关的紧缩限制性格局。为清楚起见，将PDF模型预测拟合叠加在图像上。传统的回声参数（E 峰 ，A ...

Watch this Scientific Journal Video about Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism at JoVE.

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism

全球舒张功能通过静脉流的通过参数化舒张形式主义运动学建模为基础的定量分析

准确，因果关系为基础的全球舒张功能的定量已经被通过参数化舒张肺静脉流灌装（PDF格式）形式主义的运动造型为基础的分析来实现的。 PDF生成唯一的僵硬，放松和负载参数和阐发了“新”的生理，同时提供功能障碍的敏感和特异的指标。

Quantitative cardiac function assessment remains a challenge for physiologists and clinicians.&#160;Although historically invasive methods have comprised ...

Research

JoVE Journal

Bioengineering

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价

Non-viral gene delivery using polymeric nanoparticles has emerged as an attractive approach for gene therapy to treat genetic diseases1 and as a ...

科研

生物工程

Investigating the Three-dimensional Flow Separation Induced by a Model Vocal Fold Polyp

研究模型声乐折叠聚合物引起的三维流量分离

The fluid-structure energy exchange process for normal speech has been studied extensively, but it is not well understood for pathological conditions. ...

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

电动细胞基质阻抗传感的内皮细胞增殖，屏障功能的定量和能动性

Electric Cell-substrate Impedance Sensing (ECIS) is an in vitro impedance measuring system to quantify the behavior of cells within adherent cell layers. ...

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

木质素下调<em&gt;玉米</em&gt;通过dsRNAi和Klason木质素分析

To facilitate the use of lignocellulosic biomass as an alternative bioenergy resource, during biological conversion processes, a pretreatment step is ...

Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin

用 Imagej 插件对小梁骨的外边界辅助分割和量化

Micro-computed tomography (micro-CT) is routinely used to assess bone quantity and trabecular microstructural properties in small animals under different ...

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate LAL Assays

用利穆鲁布莱巴细胞裂解液 (lal) 法检测纳米配方中的内毒素

When present in pharmaceutical products, a Gram-negative bacterial cell wall component endotoxin (often also called lipopolysaccharide) can cause ...

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels

三维水凝胶中全球细胞基质金属蛋白酶和代谢活性的测定

Three-dimensional (3D) cell culture systems often more closely recapitulate in vivo cellular responses and functions than traditional two-dimensional (2D) ...

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures

从癌细胞培养物中收集的介质光谱分析的选定金核素的同步量化

The kynurenine pathway and the tryptophan catabolites called kynurenines have received increased attention for their involvement in immune regulation and ...

The Quantification of Injectability by Mechanical Testing

机械测试的注入性量化

Injectable biomaterials are becoming increasingly popular for the minimally invasive delivery of drugs and cells. These materials are typically more ...