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18.3K Views
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12:55 min
January 17th, 2015
DOI :
10.3791/51492-v
Chapters
0:05
Title
2:17
Preparing Cells for Phage Selection
3:28
Library Pre-clearance and Antigen-phage Incubation
5:45
Phage Elution and Amplification
8:03
Characterization by Pooled ELISA
10:22
Anticipated Results and Troubleshooting
12:18
Conclusion
Summary
一种方法是描述视觉伴奏用于同时进行可扩展,高吞吐量选择从噬菌体展示的组合合成的抗体文库针对数百抗原。使用这种平行的方式,我们已经分离,表现出对于不同的抗原有功能在标准免疫高亲和力和特异性的抗体片段。
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