Anthony Kossiakoff has not added Biography.
If you are Anthony Kossiakoff and would like to personalize this page please email our Author Liaison for assistance.
Structure of a phage display-derived variant of human growth hormone complexed to two copies of the extracellular domain of its receptor: evidence for strong structural coupling between receptor binding sites.
Journal of molecular biology Feb, 2002 | Pubmed ID: 11851338
Functional promiscuity of squirrel monkey growth hormone receptor toward both primate and nonprimate growth hormones.
Molecular biology and evolution Jul, 2002 | Pubmed ID: 12082127
Determination of the energetics governing the regulatory step in growth hormone-induced receptor homodimerization.
Proceedings of the National Academy of Sciences of the United States of America Feb, 2003 | Pubmed ID: 12552121
The first semi-synthetic serine protease made by native chemical ligation.
Protein expression and purification Jun, 2003 | Pubmed ID: 12767808
Site2 binding energetics of the regulatory step of growth hormone-induced receptor homodimerization.
Protein science : a publication of the Protein Society Sep, 2003 | Pubmed ID: 12930995
The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.
Journal of molecular biology Sep, 2003 | Pubmed ID: 12946357
Dissecting the binding energy epitope of a high-affinity variant of human growth hormone: cooperative and additive effects from combining mutations from independently selected phage display mutagenesis libraries.
Biochemistry May, 2004 | Pubmed ID: 15147191
The structural basis for biological signaling, regulation, and specificity in the growth hormone-prolactin system of hormones and receptors.
Advances in protein chemistry , 2004 | Pubmed ID: 15500861
The high- and low-affinity receptor binding sites of growth hormone are allosterically coupled.
Proceedings of the National Academy of Sciences of the United States of America Dec, 2004 | Pubmed ID: 15563602
The crystal structure of a quercetin 2,3-dioxygenase from Bacillus subtilis suggests modulation of enzyme activity by a change in the metal ion at the active site(s).
Biochemistry Jan, 2005 | Pubmed ID: 15628860
Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis.
Journal of molecular biology Apr, 2005 | Pubmed ID: 15755445
Total chemical synthesis and X-ray crystal structure of a protein diastereomer: [D-Gln 35]ubiquitin.
Angewandte Chemie (International ed. in English) Jun, 2005 | Pubmed ID: 15834850
Shotgun alanine scanning shows that growth hormone can bind productively to its receptor through a drastically minimized interface.
The Journal of biological chemistry Jul, 2005 | Pubmed ID: 15857837
Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Protein science : a publication of the Protein Society Sep, 2005 | Pubmed ID: 16131663
The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold.
Proteins Jan, 2006 | Pubmed ID: 16287087
Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Nature chemical biology Mar, 2006 | Pubmed ID: 16446709
Crystal structure and site 1 binding energetics of human placental lactogen.
Journal of molecular biology May, 2006 | Pubmed ID: 16546209
Structure of bistramide A-actin complex at a 1.35 angstroms resolution.
Journal of the American Chemical Society Mar, 2006 | Pubmed ID: 16551075
Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.
The Journal of biological chemistry Aug, 2006 | Pubmed ID: 16762925
The role of protein dynamics in increasing binding affinity for an engineered protein-protein interaction established by H/D exchange mass spectrometry.
Biochemistry Jul, 2006 | Pubmed ID: 16834322
Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization.
Angewandte Chemie (International ed. in English) Dec, 2006 | Pubmed ID: 17099920
Exploring and designing protein function with restricted diversity.
Current opinion in chemical biology Jun, 2007 | Pubmed ID: 17500026
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Journal of molecular biology Nov, 2007 | Pubmed ID: 17825836
Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
Journal of molecular biology Nov, 2007 | Pubmed ID: 17888451
Synthetic antibodies for specific recognition and crystallization of structured RNA.
Proceedings of the National Academy of Sciences of the United States of America Jan, 2008 | Pubmed ID: 18162543
The 1.38 A crystal structure of DmsD protein from Salmonella typhimurium, a proofreading chaperone on the Tat pathway.
Proteins May, 2008 | Pubmed ID: 18175314
Toward chaperone-assisted crystallography: protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold.
Protein science : a publication of the Protein Society Jul, 2008 | Pubmed ID: 18445622
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers.
Journal of the American Chemical Society Jul, 2008 | Pubmed ID: 18598029
Understanding mechanisms governing protein-protein interactions from synthetic binding interfaces.
Current opinion in structural biology Aug, 2008 | Pubmed ID: 18638552
X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods.
Journal of the American Chemical Society Feb, 2009 | Pubmed ID: 19133782
Principal determinants leading to transition state formation of a protein-protein complex, orientation trumps side-chain interactions.
Proceedings of the National Academy of Sciences of the United States of America Feb, 2009 | Pubmed ID: 19196954
Crystal structure of full-length KcsA in its closed conformation.
Proceedings of the National Academy of Sciences of the United States of America Apr, 2009 | Pubmed ID: 19346472
Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods.
Protein science : a publication of the Protein Society Jun, 2009 | Pubmed ID: 19472324
An engineered substance P variant for receptor-mediated delivery of synthetic antibodies into tumor cells.
Proceedings of the National Academy of Sciences of the United States of America Jul, 2009 | Pubmed ID: 19549879
Role of a salt bridge in the model protein crambin explored by chemical protein synthesis: X-ray structure of a unique protein analogue, [V15A]crambin-alpha-carboxamide.
Molecular bioSystems Jul, 2009 | Pubmed ID: 19562114
Keeping signaling in check.
Structure (London, England : 1993) Mar, 2010 | Pubmed ID: 20223207
Characterization of engineered actin binding proteins that control filament assembly and structure.
PloS one , 2010 | Pubmed ID: 21103060
A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination.
Nature structural & molecular biology Jan, 2011 | Pubmed ID: 21151117
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
Nature structural & molecular biology Apr, 2011 | Pubmed ID: 21378967
Mechanism of activation gating in the full-length KcsA K+ channel.
Proceedings of the National Academy of Sciences of the United States of America Jul, 2011 | Pubmed ID: 21730186
Substance P derivatives as versatile tools for specific delivery of various types of biomolecular cargo.
Bioconjugate chemistry Jan, 2012 | Pubmed ID: 22175275
Generating conformation-specific synthetic antibodies to trap proteins in selected functional states.
Methods (San Diego, Calif.) Mar, 2013 | Pubmed ID: 23280336
Structure of active β-arrestin-1 bound to a G-protein-coupled receptor phosphopeptide.
Nature May, 2013 | Pubmed ID: 23604254
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Nature structural & molecular biology Mar, 2014 | Pubmed ID: 24487958
Cell biology. New tricks for an old dimer.
Science (New York, N.Y.) May, 2014 | Pubmed ID: 24833381
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Cell reports Jul, 2014 | Pubmed ID: 24981860
Visualization of arrestin recruitment by a G-protein-coupled receptor.
Nature Aug, 2014 | Pubmed ID: 25043026
Probing the functions of the paramyxovirus glycoproteins F and HN with a panel of synthetic antibodies.
Journal of virology Oct, 2014 | Pubmed ID: 25122782
Applications for an engineered Protein-G variant with a pH controllable affinity to antibody fragments.
Journal of immunological methods Dec, 2014 | Pubmed ID: 25450256
Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling.
Cell communication and signaling : CCS Jan, 2015 | Pubmed ID: 25589173
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved