Name: analyzing the movement of the nauplius 'artemia salina' by optical tracking of plasmonic nanoparticles
Uploaded: 2014-07-15T14:00:00
Description: Watch this Scientific Journal Video about Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles at JoVE.com

在ERC通过高级研究员格兰特HYMEM，由东风集团通过纳米系统倡议慕尼黑（NIM），并通过Sonderforschungsbereich（SFB1032）的资金支持，项目A8深表感谢。我们感谢博士亚历山大Ohlinger，博士溶胶卡雷特罗 - 帕拉西奥斯和水疗中心Nedev支持和富有成果的讨论。

1，实验装置<ol><li>使用向上向右显微镜和暗场油冷凝器用数值孔径（NA）= 1.2为暗视场照明。使用水浸物镜与100X的放大倍率和NA = 1.0颗粒的观察和捕捉。使用空气与目标10倍放大倍率和NA = 0.2遵循无节幼体的运动。 </li><li>使用光学镊子设置与1,064 nm的连续波激光器耦合到右上方显微镜。将光阱的激光功率为100毫瓦（与目标后，功率计测量）。 </li><li>使用CMOS高速相机或数码单镜头反光（DSLR）相机?...

<ol>
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暗场显微术是一种强大的工具，用于可视化的金纳米颗粒与下面的光学衍射极限的尺寸，由于金属纳米粒子的散射截面积超过它们的几何横截面（CP. 图2A）18。在镊子的设置，这种方法甚至允许区分如果仅在单个或多个金纳米颗粒被激光束俘获，因为颗粒之间的等离激元的耦合引起红移的等离子体激元共振频率15。暗视野显微镜与光学镊子的配置，因此提供了许多新的...

基于化学和生物指标的水质评价是具有根本的重要性，以获得对水生生态系统1-3的状态和环境条件的洞察力。经典方法化学水分析是基于感官特性或物理化学参数的测定。生物指标，而另一方面，是动物物种的存在和生存提供了环境条件和污染物，为发生英寸的指示作用的典型例子，一个生态系统的影响的洞察力是桡足类，一组小甲壳类水，它可以在几乎所有的水生境4,5找到。从水样品观察这些物质的活性和生存能力因此可用于获得关于一个生态系统5的整体条件的信息。桡足类的幼虫，这是所谓的无节幼体，用自己的触角节奏招（每个幼虫有三对appenda的GES在他们的头部区域）水6游泳。这招的频率和强度，从而在年龄，体格，动物7-10环境状况的直接指标。对这些标本的任何调查通常是用显微镜观察和直接计数的无节幼体的天线招完成。由于它们的大小（〜100-500微米）11，这通常要求要么逐个或定出一个单一的无节幼体在基板上做测量。 在这里，我们展示了一种新的方法利用光学被困金纳米粒子作为一种超灵敏探测器来观察桡足类幼虫的活性水样中。光镊通常由许多团体作为精细的实验工具，分子之间适用或测量力下降到piconewton范围12-14。最近，为光镊的应用范围已扩大到观察声波振动和解决NT波动在液体介质中通过监测纳米微粒的被局限在一个光学捕捉器15的运动。被浸没在液体颗粒进行布朗​​运动。内部光陷阱，然而，这项议案的一部分由一个强大的，激光诱导，梯度力衰减。因此，光学阱的刚度和颗粒内的激光束的焦点的定位可以通过将激光功率进行调整。在同一时间，也可以揭示关于俘获电位特性和分析分子与颗粒的相互作用通过在阱监测随时间变化的颗粒运动。这种方法使得可以拾取的频率，强度，和由一个移动的物体在其液体环境中产生的流体运动的方向。我们展示了如何这一般想法可以应用于以获得个体无节幼体的运动的频谱，而不要求直接干扰样品。这种实验方法引入了一个新概念，一般为水生标本的能动的行为在一个非常敏感的方式观察。有关指示生物种的观察，这可能会扩大现行方法进行水质分析，并可以适用于获得有关健康和水生生态系统的完整性信息。

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	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Microscope Zeiss Axio Scope.A1</td>
			<td style="width:127px;">Carl Zeiss</td>
			<td style="width:201px;">490035-0012-000</td>
			<td style="width:303px;">dark field illumination</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Water objective Achroplan</td>
			<td style="width:127px;">Carl Zeiss</td>
			<td style="width:201px;">440087</td>
			<td>100x magnification, NA=1.0</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Air objective Epiplan</td>
			<td style="width:127px;">Carl Zeiss</td>
			<td style="width:201px;">442934</td>
			<td>10x magnification, NA=0.2</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Dark field oil condenser</td>
			<td style="width:127px;">Carl Zeiss</td>
			<td style="width:201px;">445323</td>
			<td>NA=1.2</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;width:244px;">Cobolt Rumba CW 1064 nm DPSSL</td>
			<td style="width:127px;">Cobolt&#160;</td>
			<td style="width:201px;">1064-05-01-2000-500</td>
			<td>1064nm, CW, &#955;=1064nm, 2 Watt, TEM00</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:244px;">Beam expander</td>
			<td style="width:127px;">Edmund Optics</td>
			<td style="width:201px;"></td>
			<td>Part no. 1064 2-8X 64414</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">High Speed Camera Dimax HD</td>
			<td style="width:127px;">PCO. Germany</td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Color Camera Canon EOS 500 D&#160;</td>
			<td style="width:127px;">Canon</td>
			<td></td>
			<td>FAQ-ID: 8201395700</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Notch filter StopLine 532/1064</td>
			<td style="width:127px;">Semrock</td>
			<td style="width:201px;">A11149-711265</td>
			<td>Part no. NF01-532U</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Water&#160;</td>
			<td style="width:127px;"></td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Nauplius Artemia Salina</td>
			<td style="width:127px;"></td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:244px;">Gold colloid</td>
			<td style="width:127px;">BBInternational</td>
			<td style="width:201px;">Batch 13741&#160;</td>
			<td>Diameter 60nm</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;">MQMie Version 3.2&#160;</td>
			<td style="width:127px;">r. Michael Quinten</td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mathematica 8.0</td>
			<td style="width:127px;">Wolfram</td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Comsol Multiphysics 4.0&#160;</td>
			<td style="width:127px;">COMSOL, Inc.</td>
			<td style="width:201px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

analyzing the movement of the nauplius 'artemia salina' by optical tracking of plasmonic nanoparticles

我们展示了如何光镊可以提供一种灵敏的工具来分析由小的水生生物的运动所产生的流体振动。通过光镊举行一个单一的黄金纳米粒子作为传感器定量的水样在无节幼体幼虫（ 卤虫 ）的有节奏的运动。这是通过监测捕获纳米颗粒的时间相关的位移为无节幼体活动的结果来实现。纳米粒子的位置的傅立叶分析，然后产生一个频谱是特征观察到的物种的运动。该实验证明这种方法来测量和表征小的水生幼虫的活性而不需要直接观察它们，以获得关于相对于所述捕集粒子幼虫的位置信息的能力。总体而言，这种方法可以给在水生e实测值对某些物种的活力见识cosystem和可扩展的传统的方法，用于分析水样的范围内。

实验装置的示意图示于图1A中 。暗场配置是必要的，以光学检测为60nm的金颗粒的位移，在一个光学阱15。的1064纳米的捕集激光器中的波长被选择为保证检测器的金颗粒12,14的稳定禁闭。在显微镜的光束分离器是用来通过物镜聚焦俘获光束和一个陷波滤波器可以防止俘获激光进入实验的检测装置。无节幼体进行了变动，周围的光学被困金纳米粒子（ 图1B）?...

Watch this Scientific Journal Video about Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles at JoVE.com

Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

我们使用等离子体激元纳米颗粒的光学跟踪探测和表征水生生物的频率变动。

分析无节幼体的运动“<em&gt;卤虫</em&gt;&#39;由电浆纳米粒子的光学跟踪

We demonstrate how optical tweezers may provide a sensitive tool to analyze the fluidic vibrations generated by the movement of small aquatic organisms. A ...

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Engineering

Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

Fabricating Metamaterials Using the Fiber Drawing Method

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Metamaterials are man-made composite materials, fabricated by assembling components much smaller than the wavelength at which they operate 1. They owe ...

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Optical Trapping of Nanoparticles

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Optical trapping is a technique for immobilizing and manipulating small objects in a gentle way using light, and it has been widely applied in trapping ...

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A method for the synthesis of electroactive polymers is demonstrated, starting with the synthesis of extended conjugation monomers using a three-step ...

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Nano-patterns fabricated with extreme ultraviolet (EUV) or electron-beam (E-beam) lithography exhibit unexpected variations in size. This variation has ...

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

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Plasmonic tweezers use surface plasmon polaritons to confine polarizable nanoscale objects. Among the various designs of plasmonic tweezers, only a few ...