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DOI :
10.3791/51543-v
June 25th, 2014
Chapters
0:05
Title
1:33
Preamplifying the Vector Genome Junctions
3:19
Magnetic Separation of the PCR Products
4:39
LAM PCR Preparation
7:39
Non-restrictive LAM PCR Preparation
8:38
Exponential Amplifications and Product Clean-Up
10:41
Electrophoresis Analysis and Vector Insertion Site Analysis
11:26
Conclusion
线性扩增介导的(LAM)-PCR方法是开发,以确定基因组中的整合型病毒载体的精确位置的方法。该技术已经发展成为学习中的基因治疗的患者中,新颖的矢量技术,T-细胞的多样性,癌症干细胞模型等生物安全克隆动力学的优越方法
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