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DOI :
10.3791/51553-v
•
11:16 min
July 12th, 2014
Chapters
0:05
Title
1:23
Protein Pulldowns: Preparation of Transiently Transfected Cells
2:50
Equilibration of the HaloLink Resin
4:01
Binding and Washing of Fusion Complexes
6:21
SDS Elution for Denaturing Gels, Western Blots, or Mass Spectrometry
7:07
Cellular Imaging of Fluorescently Labeled Fusion Proteins Using a Cytoplasmic and Nuclear Permeable Ligand
8:48
Results: Using HaloTag Technology to Determine Protein Interactions and Localization
10:47
Conclusion
のHaloTag技術は、哺乳動物細胞から両方の小規模および大規模なタンパク質複合体の単離を大きな成功を示している多機能技術です。ここでは、既存の代替案と比較してこの技術の利点を強調し、真核生物の細胞内のタンパク質機能の多くの側面を研究するために、その有用性を実証する。
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