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DOI :
10.3791/51554-v
•
9:32 min
September 9th, 2014
Chapters
0:05
Title
1:27
Isolating Monocytes
4:29
Differentiation of Monocytes to Macrophages
5:39
Macrophage Harvest
7:09
Macrophage Yields and Characterization
8:52
Conclusion
我们提出了一个简单而有效的协议对人类巨噬细胞的产生。血沉棕黄层用双密度梯度离心法处理,并分离单核细胞,然后分化为巨噬细胞中的特氟龙涂层的细胞培养袋。这最大限度地提高巨噬细胞的产量,并促进细胞收集在后续实验。
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