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DOI :
10.3791/51582-v
•
8:56 min
July 5th, 2014
Chapters
0:05
Title
1:11
Two-electrode Voltage-clamp Experiments
2:41
Measurement of Amiloride-sensitive Whole-cell Currents
4:17
Biotinylation Assay and Detection of ENaC Cleavage Fragments at Cell Surface by Western Blot
7:08
Results: Proteolytic Activation of the Epithelial Sodium Channel
8:09
Conclusion
上皮钠通道(ENaC)的异源表达在非洲爪蟾的蛋白水解活化蟾卵母细胞可通过将当前测量与生物素化的方法,调查的离子通道的酶切产物在细胞表面的外观来证明。功能重要的切割位点可通过使用位点定向诱变来识别。
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