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DOI :
10.3791/51583-v
•
7:35 min
June 9th, 2014
Chapters
0:05
Title
1:37
Expression of Elastin-like Polypeptides (ELPs)
2:22
Purification of ELPs by Inverse Transition Cycling
4:17
Removal of Contaminants from ELP through Subsequent Rounds of ITC
5:22
Characterization of ELP Tt by Temperature-programmed Turbidimetry
6:16
Results: Characterization of ELPs Purified from E. coli through ITC
7:13
Conclusion
弹性蛋白样多肽是刺激 - 反应的生物聚合物与应用,从重组蛋白纯化给药。这个协议描述了来自大肠杆菌的使用其低临界溶解温度的相变行为作为一个简单的替代色谱弹性蛋白样多肽和其肽或蛋白质融合的纯化和表征。
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