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DOI :
10.3791/51597-v
April 30th, 2014
Chapters
0:05
Title
1:23
Tissue Digestion and hACL Isolation
2:16
Cellular Expansion, Freezing and Thawing
3:26
Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
4:22
Scanning Electron Microscopy (SEM)
5:54
Immunofluorescence Staining
7:01
Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
8:02
Conclusion
对于未来的应用作为一个补丁来修复前交叉韧带(ACL)的部分撕裂,人类的ACL的细胞从内重建手术, 体外培养扩增和生长在组织工程支架材料获得的组织分离。然后进行细胞粘附和形态学进行确认的生物相容性支架上表面。
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