Name: comprehensive analysis of transcription dynamics from brain samples following behavioral experience
Uploaded: 2014-08-26T13:00:00
Description: Watch this Scientific Journal Video about Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience at JoVE.com

This work has been funded by the Israel Science Foundation Grant (ISF # 393/12), Israel Centers of Research Excellence Grant (I-CORE 1796/12), German-Israel Foundation Grant (GIF # 2299-2291.1/2011) and the Marie Curie Career Integration Grant (FP7-PEOPLE-2013-CIG #618201). Initial steps in the project were funded by an AXA postdoctoral fellowship to AC. We acknowledge the generous startup funds provided by the Edmond and Lily Safra Center for Brain Sciences.
Critical reading by members of the Citri lab is greatly appreciated.

नोट: प्रोटोकॉल जेरूसलम के हिब्रू विश्वविद्यालय के पशु की देखभाल दिशा निर्देशों के बाद. ACSF समाधान के 1 तैयारी <ol><li> पानी या NaCl के उपयुक्त इसके साथ ~ 300 mOsm / एल परासारिता लाने, 1 टेबल में वर्णित ह?...

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व्यवहार मानदंड निम्नलिखित मस्तिष्क के ऊतकों से जीन अभिव्यक्ति के सफल लक्षण वर्णन पर निर्भर करता है: व्यवहार प्रतिमान के दौरान चूहों का 1) सावधानी से निपटने; ब्याज की ऊतक के 2) त्वरित और सटीक विच्छेदन; 3) आ?...

<table border="0" cellpadding="0" cellspacing="0" width="453">
	<tbody>
		<tr>
			<td>Virusol</td>
			<td>Oriek Medical</td>
			<td>J29D</td>
			<td></td>
		</tr>
		<tr>
			<td>Isoflurane, USP 100%</td>
			<td>MINRAD INC</td>
			<td>NDC 60307-110-25</td>
			<td></td>
		</tr>
		<tr>
			<td>RNeasy plus Universal Mini Kit</td>
			<td>QIAGENE</td>
			<td>73404</td>
			<td></td>
		</tr>
		<tr>
			<td>QIAshredder</td>
			<td>QIAGENE</td>
			<td>79654</td>
			<td></td>
		</tr>
		<tr>
			<td>High Capacity cDNA Reverse Transcription kit</td>
			<td>Invitrogene</td>
			<td>AB-4368814</td>
			<td></td>
		</tr>
		<tr>
			<td>TE Buffer</td>
			<td>Invitrogene</td>
			<td>1355656</td>
			<td></td>
		</tr>
		<tr>
			<td>Behaviour Chamber (MDF; 50X45cm)</td>
			<td>Self assembled</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Inner Perspex box (30X30cm)</td>
			<td>Self assembled</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>camera and video recorder</td>
			<td>Campden Inst</td>
			<td>CMD-80051</td>
			<td></td>
		</tr>
		<tr>
			<td>Media Recorder software</td>
			<td>Noldus</td>
			<td>NDS-NMR3-00M</td>
			<td></td>
		</tr>
		<tr>
			<td>Iris Scissors</td>
			<td>FST</td>
			<td>FST-14062-09</td>
			<td></td>
		</tr>
		<tr>
			<td>Sagital Brain slicer with a 0.5mm section</td>
			<td>Brain Tree Scientific</td>
			<td>BS-AL-505S</td>
			<td></td>
		</tr>
		<tr>
			<td>Bioanalyzer</td>
			<td>Agilent Technologies</td>
			<td>The Agilent 2100 Bioanalyzer</td>
			<td></td>
		</tr>
		<tr>
			<td>Thermal cycler</td>
			<td>Bio-Rad</td>
			<td>1852048</td>
			<td></td>
		</tr>
		<tr>
			<td>Inverted microspun spatula</td>
			<td>Bochem Instrument GmbH</td>
			<td>3213</td>
			<td></td>
		</tr>
		<tr>
			<td>Biomark HD Reader</td>
			<td>Fluidigm</td>
			<td>BMHD-BMKHD</td>
			<td></td>
		</tr>
		<tr>
			<td>Dynamic array Chip for 96.96gene expression</td>
			<td>Fluidigm</td>
			<td>BMK-M-96.96</td>
			<td></td>
		</tr>
	</tbody>
</table>

comprehensive analysis of transcription dynamics from brain samples following behavioral experience

मस्तिष्क और दीर्घकालिक यादों का समेकन में अनुभव की एन्कोडिंग जीन प्रतिलेखन पर निर्भर करते हैं. एन्कोडिंग अनुभव में विशिष्ट जीन के समारोह की पहचान आणविक तंत्रिका विज्ञान के मुख्य उद्देश्यों में से एक है. इसके अलावा, विशिष्ट व्यवहार के साथ परिभाषित जीन की कार्यात्मक संघ neuropsychiatric विकारों के आधार को समझने के लिए निहितार्थ हैं. मजबूत प्रतिलेखन कार्यक्रमों की प्रेरण विभिन्न व्यवहार जोड़तोड़ निम्नलिखित चूहों के दिमाग में देखा गया है. कुछ आनुवंशिक तत्व अलग व्यवहार जोड़तोड़ के बाद और अलग मस्तिष्क नाभिक में खरीदे उपयोग कर रहे हैं, ट्रांसक्रिप्शनल कार्यक्रमों समग्र उत्प्रेरण उत्तेजनाओं और वे 1,2 अध्ययन कर रहे हैं जिसमें संरचना के लिए अद्वितीय हैं. इस प्रकाशन में, एक प्रोटोकॉल व्यवहार में गड़बड़ी के जवाब में चूहों के मस्तिष्क नाभिक से मजबूत और व्यापक ट्रांसक्रिप्शनल रूपरेखा के लिए वर्णित है.प्रोटोकॉल तीव्र कोकीन अनुभव निम्नलिखित accumbens नाभिक में जीन अभिव्यक्ति की गतिशीलता के विश्लेषण के संदर्भ में प्रदर्शन किया है. विवो अनुभव में परिभाषित करने के बाद, लक्ष्य तंत्रिका ऊतक विच्छेदित है; शाही सेना शुद्धि के बाद, कई लक्ष्य जीन की व्यापक qPCR विश्लेषण के लिए प्रतिलेखन और microfluidic सरणियों का उपयोग रिवर्स. इस प्रोटोकॉल ऐसे छोटे मस्तिष्क के नमूने या यहां तक ​​कि एकल कक्षों के रूप में व्यापक विश्लेषण शुरू सामग्री की मात्रा को सीमित करने के (50-500 जीन को संबोधित) की दिशा में सक्षम है. प्रोटोकॉल कई नमूनों की समानांतर विश्लेषण (जैसे एकल कक्षों, दवा निम्नलिखित गतिशील विश्लेषण, वायरल या व्यवहार perturbations) के लिए सबसे फायदेमंद है. हालांकि, प्रोटोकॉल भी प्रोटीन या RNAseq द्वारा पूरे जीनोम के अध्ययन के लिए पहले नमूने के लक्षण वर्णन और गुणवत्ता आश्वासन के लिए सेवा करते हैं, साथ ही पूरे जीनोम के अध्ययन से प्राप्त आंकड़ों का सत्यापन कर सके.

इस प्रोटोकॉल को लागू करने से प्राप्त परिणामों की गुणवत्ता महत्वपूर्ण मापदंडों की एक संख्या पर निर्भर करता है. उचित प्रयोगात्मक नियोजन प्रयोगात्मक चूहों को न्यूनतम अशांति, में परिणाम होगा इस तरह के प?...

Watch this Scientific Journal Video about Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience at JoVE.com

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

व्यवहार अनुभव के बाद मस्तिष्क के नमूनों से ट्रांसक्रिप्शन गतिशीलता के व्यापक विश्लेषण

The encoding of experiences in the brain and the consolidation of long-term memories depend on gene transcription. Identifying the function of specific ...

Research

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Behavior

Contextual and Cued Fear Conditioning Test Using a Video Analyzing System in Mice

चूहों में एक वीडियो विश्लेषण प्रणाली का प्रयोग प्रासंगिक और cued डर कंडीशनिंग टेस्ट

The contextual and cued fear conditioning test is one of the behavioral tests that assesses the ability of mice to learn and remember an association ...

Transcranial Magnetic Stimulation for Investigating Causal Brain-behavioral Relationships and their Time Course

कारण मस्तिष्क व्यवहार रिश्तों और उनके समय पाठ्यक्रम की जांच के लिए transcranial चुंबकीय उत्तेजना

Transcranial magnetic stimulation (TMS) is a safe, non-invasive brain stimulation technique that uses a strong electromagnet in order to temporarily ...

Combined Shuttle-Box Training with Electrophysiological Cortex Recording and Stimulation as a Tool to Study Perception and Learning

धारणा और सीखने का अध्ययन करने के लिए एक उपकरण के रूप में Electrophysiological कॉर्टेक्स रिकॉर्डिंग और उत्तेजना के साथ शटल-बॉक्स प्रशिक्षण संयुक्त

Shuttle-box avoidance learning is a well-established method in behavioral neuroscience and experimental setups were traditionally custom-made; the ...

Rodent Brain Microinjection to Study Molecular Substrates of Motivated Behavior

कृंतक मस्तिष्क Microinjection आण्विक Substrates प्रेरित व्यवहार का अध्ययन करने के लिए

Brain microinjection can aid elucidation of the molecular substrates of complex behaviors, such as motivation. For this purpose rodents can serve as ...

Vagus Nerve Stimulation as a Tool to Induce Plasticity in Pathways Relevant for Extinction Learning

एक उपकरण के रूप में Vagus तंत्रिका उत्तेजना विलुप्त होने सीखने के लिए प्रासंगिक रास्ते में Plasticity प्रेरित करने के लिए

Extinction describes the process of attenuating behavioral responses to neutral stimuli when they no longer provide the reinforcement that has been ...

Assessing Working Memory in Children: The Comprehensive Assessment Battery for Children &#8211; Working Memory (CABC-WM)

बच्चों में वर्किंग मेमोरी का आकलन: बच्चों के लिए व्यापक आकलन बैटरी - वर्किंग मेमोरी (सीएबीसी-डब्ल्यूएम)

The Comprehensive Assessment Battery for Children - Working Memory (CABC-WM) is a computer-based battery designed to assess different components of ...

Detecting Behavioral Deficits in Rats After Traumatic Brain Injury

दर्दनाक मस्तिष्क चोट के बाद चूहों में व्यवहार घाटे का पता लगाने

With the increasing incidence of traumatic brain injury (TBI) in both civilian and military populations, TBI is now considered a chronic disease; however, ...

An Automated T-maze Based Apparatus and Protocol for Analyzing Delay- and Effort-based Decision Making in  Free Moving Rodents

एक स्वचालित टी-भूलभुलैया आधारित तंत्र और प्रोटोकॉल देरी का विश्लेषण करने के लिए और प्रयास आधारित मुक्त चलती कुतर में बनाने का निर्णय

Many neurological and psychiatric patients demonstrate difficulties and/or deficits in decision making. Rodent models are helpful to produce a deeper ...

Automated, Long-term Behavioral Assay for Cognitive Functions in Multiple Genetic Models of Alzheimer's Disease, Using IntelliCage

स्वचालित, अल्जाइमर रोग के कई आनुवंशिक मॉडल में संज्ञानात्मक कार्यों के लिए दीर्घकालिक व्यवहार परख, IntelliCage का उपयोग

Multiple factors&#8212;such as aging and genes&#8212;are frequently associated with cognitive decline. Genetically modified mouse models of cognitive ...