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DOI :
10.3791/51679-v
August 1st, 2014
Chapters
0:05
Title
1:49
In Situ Staining of Cerebral Sections with MHC Class II (IAs)/PLP 139-151 Dextramers
6:28
Analysis of Dextramer+ (dext+) CD4+ T Cells by Laser Scanning Confocal Microscopy (LSCM)
10:50
Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers
12:29
Conclusion
通过直接染色与主要组织相容性复合物Ⅱ类dextramers检测自身反应性CD4 + T细胞在大脑和心脏的协议已经本报告中进行了描述。为综合分析,一种可靠的方法来枚举抗原特异性CD4 + T细胞在原位的频率也被设计出来。
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