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DOI :
10.3791/51679-v
August 1st, 2014
Chapters
0:05
Title
1:49
In Situ Staining of Cerebral Sections with MHC Class II (IAs)/PLP 139-151 Dextramers
6:28
Analysis of Dextramer+ (dext+) CD4+ T Cells by Laser Scanning Confocal Microscopy (LSCM)
10:50
Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers
12:29
Conclusion
主要組織適合性複合体クラスII dextramersとの直接染色により脳及び心臓における自己反応性CD4 T細胞を検出するためのプロトコルは、このレポートに記載されている。総合的な分析のために、 その場での抗原特異的CD4 + T細胞の頻度を列挙するための信頼できる方法も考案されている。
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