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DOI :
10.3791/51683-v
•
12:54 min
September 4th, 2014
Chapters
0:05
Title
1:52
Labeling and Injection of HSPCs
3:21
Preparing the Mouse for Imaging
5:20
In Vivo Imaging: High-resolution Stacks and Time-lapse Acquisition
11:25
Results: In Vivo 4D Imaging of HSPCs in Mouse Calvarium
12:16
Conclusion
造血干细胞和祖细胞(HSPCs)和骨髓龛之间的相互作用的性质知之甚少。定制的硬件修改和多步获取协议允许使用双光子共焦显微术图像体外骨髓区域内驻留标记HSPCs,跟踪相互作用和运动。
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