Name: transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation
Uploaded: 2014-07-23T15:00:00
Description: Watch this Scientific Journal Video about Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation at JoVE.com

这项工作是支持的，部分由一个津贴从美国国立卫生研究院（RO1 HL098228）到CKB。

注：所有动物的程序批准了全国儿童医院实验动物管理和使用委员会。 1，肺动脉心脏瓣膜收获从心脏瓣膜供体小鼠<ol><li>高压灭菌所有的外科手术工具手术前：1倍罚款剪刀，3微型镊子，微型2倍血管钳，1x夹紧应用镊子，1个微型针器，1个弹簧剪刀，1卷收器。 </li><li> 6-8周龄雌性C57BL / 6小鼠作为肺动脉心脏瓣膜捐助。从笼中取出小鼠并称重，然后用安乐死氯胺酮/甲苯​​噻...

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	<li>Polito, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+morbidity+and+mortality+in+very+preterm+VLBW+infants+with+congenital+heart+disease.">Increased morbidity and mortality in very preterm VLBW infants with congenital heart disease.</a> Intens Care Med. 39, 1104-1112 (2013).</li><li>Wren, C., Reinhardt, Z., Khawaja, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Twenty+year+trends+in+diagnosis+of+life+threatening+neonatal+cardiovascular+malformations.">Twenty year trends in diagnosis of life threatening neonatal cardiovascular malformations.</a> Arch Dis Child Fetal. 93, F33-F35 (2008).</li><li>Vacanti, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beyond+transplantation+Third+annual+Samuel+Jason+Mixter+lecture.">Beyond transplantation Third annual Samuel Jason Mixter lecture.</a> Archives of surgery. 123, 545-549 (1960).</li><li>Tudorache, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+replacement+of+aortic+heart+valves+with+tissue-engineered+grafts.">Orthotopic replacement of aortic heart valves with tissue-engineered grafts.</a> Tissue engineering Part A. 19, 1686-1694 (2013).</li><li>van Geldorp, M. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patient+outcome+after+aortic+valve+replacement+with+a+mechanical+or+biological+prosthesis+weighing+lifetime+anticoagulant+related+event+risk+against+reoperation+risk.">Patient outcome after aortic valve replacement with a mechanical or biological prosthesis weighing lifetime anticoagulant related event risk against reoperation risk.</a> The Journal of thoracic and cardiovascular surgery. 137, 881-886 (2009).</li><li>El Oakley, R., Kleine, P., Bach, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choice+of+prosthetic+heart+valve+in+today's+practice.">Choice of prosthetic heart valve in today's practice.</a> Circulation. 117, 253-256 (2008).</li><li>Quinn, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+Models+for+Bench+to+Bedside+Translation+of+Regenerative+Cardiac+Constructs.">Animal Models for Bench to Bedside Translation of Regenerative Cardiac Constructs.</a> Progress in Pediatric cardiology. 35, (2013).</li><li>Shinoka, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineering+heart+valves+valve+leaflet+replacement+study+in+a+lamb+model.">Tissue engineering heart valves valve leaflet replacement study in a lamb model.</a> The Annals of thoracic surgery. 60, S513-S516 (1995).</li><li>Sodian, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineering+of+heart+valves+in+vitro+experiences.">Tissue engineering of heart valves in vitro experiences.</a> The Annals of thoracic surgery. 70, 140-144 (2000).</li><li>Shinoka, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineered+heart+valves.+Autologous+valve+leaflet+replacement+study+in+a+lamb+model.">Tissue engineered heart valves. Autologous valve leaflet replacement study in a lamb model.</a> Circulation. 94, II164-II168 (1996).</li><li>Sodian, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+biodegradable+three+dimensional+matrices+for+tissue+engineering+of+heart+valves.">Evaluation of biodegradable three dimensional matrices for tissue engineering of heart valves.</a> ASAIO journal (American Society for Artificial Internal Organs. 46, 107-110 (1992).</li><li>Sodian, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+in+vivo+experience+with+tissue-engineered+trileaflet+heart+valves).">Early in vivo experience with tissue-engineered trileaflet heart valves).</a> Circulation. 102, III22-III29 (2000).</li></ol>

死亡率此程序是接近20％，这主要是由出血在PV移植部位吻合捐助主动脉到受体腹主动脉造成的。在大多数情况下，死亡率显著减小后48小时的手术。存活小鼠表现出通过植入光伏强劲的心脏跳动和血液的流动。整个过程需要四个小时有经验的外科医生微。这将需要大约250小鼠掌握的技术。异位心脏移植是比较直截了当相比，光伏植入的供体心脏。其中一个成功的高压移植中最关键的步骤是收获从?...

先天性心血管缺陷的婴儿死亡率在西方世界1,2的主要原因之一。其中，肺动脉瓣狭窄和主动脉瓣二瓣缺陷是一个经常出现的形式3。心脏瓣膜置换手术是修复手术的常规选择;然而，并发症，包括狭窄的心脏瓣膜的钙化，以及终身抗凝药物的依赖是慢性疾病和死亡4-7一个显著来源。此外，缺乏增长潜力，需要重新进行手术，这进一步增加了这些年轻患者4,8,9的死亡率。 在试图开发一种功能置换心脏瓣膜的增长潜力，Shinoka 等。种子自体细胞到可生物降解的合成心脏瓣膜8。合成阀转换成一个原生心脏瓣膜状结构与增长电位人。初步大型动物的研究表明使用这种方法来创建一个功能心脏瓣膜10的可行性。然而，长期植入的研究表明耐用性差，由于该阀neotissue导致心脏瓣膜变窄的逐渐增厚。从Sodian工作等使用的Shinoka方法，但最终换成了PGA矩阵，可生物降解弹性体，这给了组织工程瓣膜的生物力学特性构建一个更符合生理轮廓9,11,12。在体内研究中，尽管植入的成功，一个融合的内皮细胞衬里没有形成这可能限制了长期的成功这个支架12。 为了合理设计改进的第二代人造心脏瓣膜，建立心脏瓣膜移植的小鼠模型来研究underlyin的细胞和分子机制克neotissue形成，瓣膜增厚，管腔狭窄和发展。小鼠模型提供了分子试剂繁多，包括转基因，这是不是在其他物种7一应俱全。在这种心脏瓣膜移植模型， 离体同源的肺心脏瓣膜置换首先执行;然后与植入心脏瓣膜的心脏被植入异位成利用显微外科技术一同源主机。通过此模型，心脏瓣膜置换，无需体外循环。 在本文中，心脏瓣膜收获的详细说明，供体心脏的准备工作，心脏瓣膜移植和异位心脏移植模型描述。结果表明，连续的心跳从供体心脏，这是独立于接收者的心跳。通过植入肺动脉瓣的血流使用高频超声系统用脉冲波执行测量ppler。

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transplantation of pulmonary valve using a mouse model of heterotopic heart transplantation

组织工程心脏瓣膜，特别是脱细胞瓣膜，也开始获得动力在临床使用重建手术的结果好坏参半。然而，neotissue发展，阀增厚和狭窄发展的细胞和分子机制尚未广泛研究。要回答上述问题，我们开发了一个小鼠异位心脏瓣膜移植模型。心脏瓣膜是从阀供体小鼠收获移植到心脏供体小鼠。用一个新的阀的心脏异位移植到受体小鼠。移植心脏表明自己的心跳，独立收件人的心跳。使用高频超声系统用脉冲多普勒血流进行定量。通过植入肺动脉瓣流量呈正向流量以最小的反流和洪峰流量接近100毫米/秒。心脏瓣膜移植的这个小鼠模型是highlÝ通用性，因此它可以被修改和调整以提供不同的血流动力学的环境和/或可用于各种转基因小鼠来研究neotissue发展在组织工程心脏瓣膜。

图1说明了心脏瓣膜移植模型采用异位心脏移植的原理图。心脏瓣膜是从供体心脏收获，并从第二供体小鼠植入到心脏。然后用新的心脏瓣膜的心脏被植入到受体小鼠的腹部。 图2显示了植入心脏的插图上腹部空间（A），心脏移植后（B）的权利，移植后5分钟。在剔除对主动脉和下腔静脉的两侧缝线，心脏开始后打1-2分钟，成为平克与更多的血液?...

Watch this Scientific Journal Video about Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation at JoVE.com

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

为了了解neotissue形成和狭窄的发展在组织工程心脏瓣膜相关的细胞和分子机制，开发了异位心脏瓣膜移植的小鼠模型。使用异位心脏移植术肺动脉心脏瓣膜移植到收件人。

肺动脉瓣使用异位心脏移植小鼠模型移植

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Research

JoVE Journal

Medicine

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

用改进的袖套法小鼠颈部心脏移植模型

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

科研

医学

Murine Heterotopic Heart Transplant Technique

小鼠异位心脏移植技术

It is now over forty years since this technique was first reported by Corry, Wynn and Russell. Although it took some years for other labs to become ...

Rat Heterotopic Abdominal Heart/Single-lung Transplantation in a Volume-loaded Configuration

大鼠异位心脏腹部/在卷装配置单肺移植

Herein, we describe a novel technique for heterotopic abdominal heart-lung transplantation (HAHLT) in rats. The configuration of the transplant graft ...

Heterotopic Cervical Heart Transplantation in Mice

颈椎异位心脏移植小鼠

The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies ...

A Novel Microsurgical Model for Heterotopic, En Bloc Chest Wall, Thymus, and Heart Transplantation in Mice

一种新的显微模型异位，整块胸壁，胸腺，和心脏移植小鼠

Exploration of novel strategies in organ transplantation to prolong allograft survival and minimizing the need for long-term maintenance immunosuppression ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

A Simplified Model for Heterotopic Heart Valve Transplantation in Rodents

啮齿动物异位心脏瓣膜移植的简化模型

There is an urgent clinical need for heart valve replacements that can grow in children. Heart valve transplantation is proposed as a new type of ...

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

一种猪异位心脏移植方案，用于向心脏同种异体移植物提供治疗药物

Cardiac transplantation is the gold standard treatment for end-stage heart failure. However, it remains limited by the number of available donor hearts ...

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

一种改进的袖带技术用于小鼠颈椎异位心脏移植模型

Cardiac allograft rejection limits the long-term survival of patients after heart transplantation. A mouse heart transplantation model is ideal for ...

A Heterotopic Mouse Model for Studying Laryngeal Transplantation

用于研究喉移植的异位小鼠模型

Laryngeal heterotopic transplantation, although a technically challenging procedure, offers more scientific analysis and cost benefits compared to other ...