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DOI :
10.3791/51752-v
July 14th, 2014
Chapters
0:05
Title
1:21
Spheroplast Preparation
3:07
Nanogold Uptake
3:44
Fixation and Sectioning
4:42
Silver Enhancement for Nanogold Particle Visualization
8:17
Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
9:09
Conclusion
酵母、 サッカロマイセス·セレビシエは 、エンドソーム系の生合成と機能を調節する遺伝子を同定し、研究するための重要なモデル生物となっている。ここでは、超微細構造の研究のためのエンドソーム区画の特異的標識のための詳細なプロトコルを提示する。
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