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DOI :
10.3791/51766-v
•
11:26 min
August 14th, 2014
Chapters
0:05
Title
1:59
Plating and Maintenance of Human Umbilical Vein Endothelial Cells
4:00
Polymorphonuclear Leukocyte Isolation Using Percoll Gradients
6:36
Labeling of Endothelial Junctional VE-cadherin and PECAM-1 and Performing the PMN TEM Assay Under Flow
9:24
Results: Visualization of the Dynamics of VE-cadherin and PECAM-1 During Neutrophil TEM
10:59
Conclusion
白血球は、傍細胞または経細胞経路を用いて内皮単層を横切る。私たちは、内因性の接合部の分布がVE-カドヘリンおよびPECAM-1を白血球経内皮移行中に生理的な流れの下2移住ルートを区別するために従うように、単純なアッセイを開発した。
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