Name: human skeletal muscle biopsy procedures using the modified bergstr&#246;m technique
Uploaded: 2014-09-10T14:00:00
Description: Watch this Scientific Journal Video about Human Skeletal Muscle Biopsy Procedures Using the Modified BergstrÃƒÂ¶m Technique at JoVE.com

자금 지원 선언 없습니다.

설명 된 골격 근육 절차는 애팔 래 치아 주립 대학위원회 (Institutional Review Board)의 지침을 따른다. NOTE : 팀 연산자 (박사 또는 생검 기법에서 숙련 MD), 및 적어도 하나의 구성되지만 이상적으로, 세 개 이상의 기술자. 오퍼레이터는 전도성 및 골격근 생검 절차의 모든 측면을 감독하고있다. 한 기술자 (기술자 # 1) 근육​​ 추출의 모든 측면을 돕는 오퍼레이터 즉시 책임이있다....

<ol>
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이 동영상은 ASU 인간 성능 연구소에서 사용되는 골격 근육 생검 절차의 단계별 요약을 제공합니다. 작은 수정 사항이 절차는, 지난 13 년 동안 약 1600 근육 조직 검사를 수집하는 데 사용되었다. 근육 생검 샘플은 중요한 연구의 발견으로 이어지는, 스포츠 영양 기초 연구에 중요한 자료를 제공하고 있습니다. 수정 된 버그 스트롬 골격 근육 조직 검사 기법을 수행 할 때주의해...

경피적 또는 &#39;세미 오픈&#39;조직 검사 기술은 인간의 환자와 연구 대상에서 골격 근육 조직 표본을 얻기 위해 사용된다. 뒤 시엔 느 (1,806에서 1,875 사이)은 경피적 생검 일을 통해 생활 과목에서 골격 근육을 얻기 위해 투관침으로 바늘을 구성하는 것이 첫번째로 적립됩니다. 1960 년대에 버그 스트롬은 뒤 시엔 느 2-4에서 기재 한 것과 유사한 경피적 생검 바늘을 도입했다. 20 년 후, 에반스 등. 5 버그 스트롬 바늘의 절단 투관침을 통해 흡입을 적용하여 기술을 수정했습니다. 본 변형 예에서는 5 배 6,7 티슈 3의 수율을 향상시킬 수 있고, 생물 의학 연구와 임상 설정에서 사용된다. 이 기술은 가지고 있으며, 근육 병증의 진단 및 골격 근육의 구조와 기능에 대한 우리의 이해를 지속적으로 육성 해 나갈 것입니다. 경피적 근육 생검 기법이다 straightforward. 제대로 할 때, 무균 기술에 대한 엄격한 준수와 관련된 위험을 최소화합니다. 근육 조직 검사 절차는 연구 프로젝트에 대한 임상 도구로 간주된다. 일부 교육 기관은 근육 조직 검사를 얻기 위해, 직접 의사의 감독으로, 박사 학위와 교수 연구원을 교육 훈련을 받았 다른 기술을 수행하는 보드 인증 의사를 필요로합니다. ASU 조직 검사 팀은 이전에는 성공적으로 십삼년 8-15 중 1600여 근육 생검을 수행하고있다. 이 동영상의 목적은 인체의 측광 근 lateralis에서 골격근 조직 샘플을 얻기 위해 수정 된 버그 스트롬 바늘 경피적 근육 생검 기법 5를 설명한다.

<table border="0" cellpadding="0" cellspacing="0" width="969">
	<tbody>
		<tr height="24">
			<td height="24" style="height:24px;width:439px;">Name of Material / Equipment</td>
			<td style="width:185px;">Company</td>
			<td style="width:141px;">Catalog Number</td>
			<td style="width:204px;">Comments /&#160; Description</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Bergstr&#246;m biopsy needle, 5 x 100mm</td>
			<td style="width:185px;">Stille Surgical Inc.</td>
			<td>119-29187-50</td>
			<td>1 ea</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Durasorb blue pad</td>
			<td>Fisher</td>
			<td>22-031-340</td>
			<td>case of 300</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Prep Razor Disposable</td>
			<td>Moore Medical</td>
			<td>89760</td>
			<td>1 ea</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Shave Cream</td>
			<td>Moore Medical</td>
			<td>92869</td>
			<td>1 can</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Alcohol Swab</td>
			<td>Moore Medical</td>
			<td>98721</td>
			<td>box/200</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Povidone-iodine Swabsticks (3 pack)</td>
			<td>Moore Medical</td>
			<td>90691</td>
			<td>pack of 50</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Face masks</td>
			<td>Fisher</td>
			<td>19-039-690</td>
			<td>case of 50</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Sterile Gloves; sz 6.5</td>
			<td>Moore Medical</td>
			<td>68128</td>
			<td>box of 50 pairs</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Sterile Gloves; sz 7.0</td>
			<td>Moore Medical</td>
			<td>68129</td>
			<td>box of 50 pairs</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Sterile Gloves; sz 8.5</td>
			<td>Moore Medical</td>
			<td>68132</td>
			<td>box of 50 pairs</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Fenestrated Towel Drape</td>
			<td>Moore Medical</td>
			<td>92712</td>
			<td>case of 50</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Lidocaine (1% HCl, w/o Epi</td>
			<td>Dealmed</td>
			<td>427902</td>
			<td>30cc vial</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Ethyl Chloride spray</td>
			<td>Dealmed</td>
			<td>386020</td>
			<td>3.5 fl. Oz. bottle</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">5 mL syringe w/ 21G needle</td>
			<td>Fisher</td>
			<td>14-827-48</td>
			<td>case of 100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">25G 5/8&#34; needle</td>
			<td>Fisher</td>
			<td>14-826AA</td>
			<td>case of 100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">25G 1 1/2&#34; needle</td>
			<td>Fisher</td>
			<td>14-826-49</td>
			<td>case of 100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Single-Use Scalpels&#160; w/ #11 blade</td>
			<td>Fisher</td>
			<td>0-8927-5B</td>
			<td>box of 20</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">60 mL Syringe</td>
			<td>Fisher</td>
			<td>13-689-8</td>
			<td>case of 40</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">~30 inch extension tubing</td>
			<td>Fisher</td>
			<td>14-169-7A</td>
			<td>roll of 50ft</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Metal stopcock</td>
			<td>Fisher</td>
			<td>01-290-38</td>
			<td>each</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Polypropylene tubing connector w/ tapered ends, 3.2 to 5.5 mm</td>
			<td>Fisher</td>
			<td>15-315-28A</td>
			<td>case of 100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Yellow pipette tips (1-200 &#181;L)</td>
			<td>Fisher</td>
			<td>02-681-2</td>
			<td>cut off&#160; ~15-18 mm of the tip</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Topical surgical adhesive</td>
			<td>Medline</td>
			<td>MSC091076</td>
			<td>case of 36 tubes</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Topical surgical adhesive</td>
			<td>Medline</td>
			<td>MSC091076Z</td>
			<td>box of 6 tubes</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Butterfly stitches (adhesive strips) 1/2&#34; x 4&#34; (6 per pack)</td>
			<td>Moore Medical</td>
			<td>93050</td>
			<td>box/300</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Bandadge, Strip, 3/4 x 3 in.</td>
			<td>Fisher</td>
			<td>19-027-202</td>
			<td>pack of 100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Adhesive bandage (5m rolls)</td>
			<td>Fisher</td>
			<td>19-027-768</td>
			<td>case of 36</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">1&#34; paper surgical tape</td>
			<td>Fisher</td>
			<td>19-027-761</td>
			<td>case of 12</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Sutures (4-0 reverse cutting, FS2)</td>
			<td>Moore Medical</td>
			<td>82926</td>
			<td>box of 36</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">4 x 4 sterile gauze pads</td>
			<td>Moore Medical</td>
			<td>66949</td>
			<td>pkg/50</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">2 x 2 non-sterile gauze pads</td>
			<td>Moore Medical</td>
			<td>37336</td>
			<td>pkg/200</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">3 x 4 Non-Adherent Pads</td>
			<td>Moore Medical</td>
			<td>74389</td>
			<td>bx/100</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Autoclave bags 3.5 x 9</td>
			<td>Moore Medical</td>
			<td>40390</td>
			<td>box of 200</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Autoclave bags 5.25 x 10</td>
			<td>Moore Medical</td>
			<td>40391</td>
			<td>box of 200</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Autoclave indicator tape</td>
			<td>Moore Medical</td>
			<td>31608</td>
			<td>box of 250</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Lab Coats&#8212;small</td>
			<td>Fisher</td>
			<td>23-900-520A&#160;</td>
			<td>10/pk</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Lab Coats&#8212;medium</td>
			<td>Fisher</td>
			<td>23-900-520B&#160;</td>
			<td>10/pk</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Lab Coats&#8212;large</td>
			<td>Fisher</td>
			<td>23-900-520C&#160;</td>
			<td>10/pk</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Germicidal Disposable Cloth Wipes</td>
			<td>Moore Medical</td>
			<td>62879</td>
			<td>Can/160</td>
		</tr>
	</tbody>
</table>

human skeletal muscle biopsy procedures using the modified bergstr&#246;m technique

경피적 생검 기술은 연구자와 임상의 골격 근육 조직 샘플을 수집 할 수 있습니다. 이 기술은 안전하고 매우 효과적이다. 이 동영상은 인체의 광근의 lateralis에서 골격 근육 조직 샘플을 얻기 위해 수정 된 버그 스트롬 바늘을 사용하여 경피적 생검 기술에 대해 설명합니다. 버그 스트롬 바늘 선단 절삭 블레이드 팁의 측면의 작은 개구 ( &#39;윈도우&#39;)와 내측 투관침과 캐 뉼러 외부로 구성된다. 국소 마취하고 무균 조건 하에서, 니들은 피부, 피하 조직 및 근막에 절개를 통하여 골격근에 진출한다. 다음에, 흡인 내측 투관침에인가되고, 따라서, 절단 또는 골격근 조직 샘플 패스, 외측 투관침은 골격근 조직을 흡인 외측 뉼러의 윈도우로 흡입되고, 다시 당기면, 내측 투관침 빠르게 닫혀 . 주사 바늘을 90 ° 회전(60), 그리고 또 다른 컷이 이루어집니다. 이 과정은 세 번 이상 반복 될 수있다. 이것은 여러 절단 기법은 일반적 건강한 피험자 100-200 밀리그램 이상의 샘플을 생산하고 동안, 직전에 수행 할 수 있으며, 운동이나 다른 간섭의 시합 후의. 절개 사이트의 후 조직 검사 드레싱에 따라, 환자는 일반적으로 바로 일상 생활의 활동을 재개하고 완벽하게 48 ~ 72 시간 이내에 격렬한 신체 활동에 참여할 수 있습니다. 주제는 근막의 절개를 통해 근육의 탈출증의 위험을 줄이기 위해 48 시간 동안 무거운 저항 운동을 피해야한다.

전술 한 바와 같이 근육 생검 절차가 신속하고 지속적으로 골격근 조직 샘플을 수집 할 수 있도록 연구. 건강, 체육 과목에서 전형적인 수율은 3-4 클립 단일 패스에서 200 밀리그램 이상이다. 절차는 절개 준비에 소요되는 대부분이 15 ~ 20 분, 소요. 운동 기반 연구에서 근육 샘플은 종종 복구하는 동안 수집 된 하나 또는 두 개의 샘플, 전후 운동을 가져옵니다. 이 디자인으로, 운동 후에 머리 (반대?...

Watch this Scientific Journal Video about Human Skeletal Muscle Biopsy Procedures Using the Modified BergstrÃƒÂ¶m Technique at JoVE.com

Human Skeletal Muscle Biopsy Procedures Using the Modified BergstrÃƒÆ’Ã‚Â¶m Technique

수정 된 버그 스트롬 기술을 이용하여 인간의 골격 근육 생검 절차

The purpose of this video is to present the modified Bergstr&#246;m skeletal muscle biopsy technique on human subjects.

The percutaneous biopsy technique enables researchers and clinicians to collect skeletal muscle tissue samples. The technique is safe and highly ...

Research

JoVE Journal

Medicine

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&#246;m Technique

Myo-mechanical Analysis of Isolated Skeletal Muscle

절연 골격근의 묘 - 기계 분석

To assess the in vivo effects of therapeutic interventions for the treatment of muscle disease 1,2,3, quantitative methods are needed that measure force ...

연구

의학

Skeletal Muscle Gender Dimorphism from Proteomics

Proteomics에서 골격근 성별 동종 이형

Gross contraction in skeletal muscle is primarily determined by a relatively small number of contractile proteins, however this tissue is also remarkably ...

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

인간의 기본 섬유 아세포의 유도를위한 피부 펀치 생검 절편 문화

Tissues and cell lines derived from an individual with disease are ideal sources to study disease-related cellular phenotypes. Patient-derived fibroblasts ...

Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice

경골 신경 절개 - 마우스의 탈 신경에 의한 골격근의 위축에 대한 표준 모델

The tibial nerve transection model is a well-tolerated, validated, and reproducible model of denervation-induced skeletal muscle atrophy in rodents. ...

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

수정 커프 기술을 사용하여 쥐의 자궁 심장 이식 모델

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

인간 혈청에있는 N-메틸-D-아스파 라진 산염 수용체 또는 도파민 2 수용체에 대한 항체를 검출하는 높은 처리량 유동 세포 계측법 세포 기반 분석

Over the recent years, antibodies against surface and conformational proteins involved in neurotransmission have been detected in autoimmune CNS diseases ...

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

경피적 바늘 생검에 의해 획득 골격근 조직에서 고립 된 미토콘드리아의 준비 및 호흡계 평가

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

골격근 질환의 정량적 자기 공명 영상

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

사람의<em&gt; 광근의 Lateralis</em&gt; 웨일 - Blakesley Conchotome를 사용하여 골격 근육 생검

Percutaneous muscle biopsy using the Weil-Blakesley conchotome is well established in both clinical and research practice. It is a safe, effective and ...

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

인-31 자기 공명 분광학 : 측정을위한 도구<em&gt; 생체</em인간의 골격 근육에&gt; 미토콘드리아 산화 적 인산화 능력

Skeletal muscle mitochondrial oxidative phosphorylation (OXPHOS) capacity, which is critically important in health and disease, can be measured in vivo ...