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09:39 min
August 21st, 2014
DOI :
10.3791/51814-v
Chapters
0:05
Title
1:04
Sample Collection
1:46
RNA Extraction Analysis
3:04
Acrylamide Gel and Sample Preparation
3:58
Electrophoretic Fractionation
4:41
Gel Blotting
6:03
Probe Synthesis, Membrane Crosslinking, and Prehybridization
7:27
Membrane Hybridization, Washing, and Signal Detection
8:20
Results: Improved RNA Detection Sensitivity
9:06
Conclusion
Summary
本刊物的目的是可视化和探讨手术步骤,从果蝇的RNA提取的增强Northern杂交协议的果蝇胚胎,细胞和组织。这个协议是用于有效地检测小RNA种类是特别有用的。
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