Institut de Génétique et de Biologie Moléculaire et Cellulaire

3 ARTICLES PUBLISHED IN JoVE

Biology

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

¹Département de Génomique Fonctionnelle et Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²Center for Life NanoScience, Istituto Italiano di Tecnologia

The aim of this publication is to visualize and discuss the operative steps of an Enhanced Northern Blot protocol on RNA extracted from Drosophila melanogaster embryos, cells, and tissues. This protocol is particularly useful for the efficient detection of small RNA species.

Biochemistry

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Shilpy Joshi¹, Vanessa Ueberschlag-Pitiot¹, Daniel Metzger¹, Irwin Davidson¹

¹Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire

A novel protocol for the preparation of chromatin from adult mouse skeletal muscle adapted to the study of gene regulation in muscle fibers by chromatin immunoprecipitation is presented.

Developmental Biology

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

Renee Wei-Yan Chow^*1,2,3,4, Paola Lamperti^*1,2,3,4, Emily Steed^1,2,3,4, Francesco Boselli^1,2,3,4, Julien Vermot^1,2,3,4

¹Institut de Génétique et de Biologie Moléculaire et Cellulaire, ²UMR7104, Centre National de la Recherche Scientifique, ³U964, Institut National de la Santé et de la Recherche Médicale, ⁴Université de Strasbourg

This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development.