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DOI :
10.3791/51814-v
August 21st, 2014
Chapters
0:05
Title
1:04
Sample Collection
1:46
RNA Extraction Analysis
3:04
Acrylamide Gel and Sample Preparation
3:58
Electrophoretic Fractionation
4:41
Gel Blotting
6:03
Probe Synthesis, Membrane Crosslinking, and Prehybridization
7:27
Membrane Hybridization, Washing, and Signal Detection
8:20
Results: Improved RNA Detection Sensitivity
9:06
Conclusion
이 책의 목적은 시각화 및 초파리에서 추출한 RNA에 향상된 북부 얼룩 프로토콜의 동작 단계를 논의 배아, 세포 및 조직을 melanogaster하는 것입니다. 이 프로토콜은 작은 RNA 종의 효율적인 검출에 특히 유용하다.
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