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DOI :
10.3791/51823-v
•
10:30 min
October 15th, 2014
Chapters
0:05
Title
1:46
Isolation of Atrial and Ventricular Myocytes from the Mouse Heart
4:09
Imaging of Transverse-axial Tubule System (TATS) Membrane Structures in Living Myocytes
5:41
Analysis of the TATS Membrane Network and its Components
8:23
Results: Representative TATS Component Orientations for Ventricular and Atrial Myocytes
9:26
Conclusion
在心肌细胞,管式膜结构形成细胞内的网络。我们描述了优化方案从小鼠心脏肌细胞I)的隔离,包括质量控制,二)活细胞染色国家的最先进的荧光显微镜,及iii)直接图像分析来量化成分的复杂性和膜细胞的可塑性网络。
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