Name: murine corneal transplantation: a model to study the most common form of solid organ transplantation
Uploaded: 2014-11-17T13:00:00
Description: Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

The authors would like to thank the many individuals who have worked on and perfected this technique and have been responsible for the generation of many manuscripts both in this lab and others. This work was supported by National Institutes of Health Grant EY12707 (PMS) and an unrestricted grant from Research to Prevent Blindness to Department of Ophthalmology.

REMARQUE: Tous les animaux utilisés dans cette procédure sont traités conformément à l&#39;Association pour la recherche en vision et déclaration d&#39;ophtalmologie pour l&#39;utilisation d&#39;animaux dans ophtalmique et Vision Research ainsi que les lignes directrices établies par le comité de surveillance de l&#39;animal à l&#39;Université de Saint Louis. NOTE: Tous les instruments et solutions chirurgicales sont stérilisés avant la chirurgie pour limiter l&#39;infection microbienne de l&#39;œil....

<ol>
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R., Archer, B., Ambati, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascular+Endothelial+Growth+Factor+Receptor+1+morpholino+increases+graft+survival+in+a+murine+penetrating+keratoplasty.">Vascular Endothelial Growth Factor Receptor 1 morpholino increases graft survival in a murine penetrating keratoplasty.</a> Invest. Ophthalmol. Vis. Sci. 53, 8458-8471 (2012).</li><li>Kim, H. K., Choi, J. A., Uehara, H., Zhang, X., Ambati, B. K., Cho, Y. 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Le modèle murin de greffe de cornée décrite ici permet à l&#39;enquêteur d&#39;étudier le rejet d&#39;allogreffe de cornée humaine dans un modèle qui permet de prédire quels sont les facteurs associés à la fois meilleur rejet 15,17,18,20, 26-30 et 21-25 acceptation de la cornée allogreffes. Contrairement à la transplantation de la cornée humaine, dans laquelle les patients reçoivent soit un traitement de stéroïde topique ou systémique pour traiter ou prévenir une ou l&#39;autre d...

La transplantation de la cornée est un des types les plus communs et succès de la transplantation réalisée chez l&#39;homme. Les raisons pour lesquelles cette intervention chirurgicale est pratiquée sont le résultat de blessures, de maladies infectieuses 1, ou d&#39;autres formes de la maladie de la cornée non-infectieuse 2. Les chiffres de l&#39;Association of America Banque d&#39;yeux indiquent que plus de 46 000 ont été réalisées en 2011 (voir le site Web à l&#39;adresse: restoresight.org/eye_banks/eye_banks.html). Une indication de son succès est que les taux de défaillance d&#39;un an pour greffes de cornée allogéniques vont de 10 à 15% et à 5 ans, le succès est au-delà de 70% 3-8. Comme de nombreuses études ont démontré le succès des allogreffes de la cornée est directement lié au fait que l&#39;oeil est un site immunologiquement privilégié. Les facteurs responsables de l&#39;état des cornées comme un site de privilège immunitaire comprennent l&#39;absence de ces deux vaisseaux sanguins et lymphatiques dans la cornée, une absence relative de cellules présentatrices d&#39;antigènes, facteurs produite par la cornée qui Suppress funtions immunitaires effectrices 9-15, faible expression de antigènes du CMH 16, et l&#39;expression de FasL 17-20. Cependant, en dépit de ces facteurs prédisposants ces greffes de succès, ils ne subissent rejet 3-7. Par conséquent, la compréhension de ces mécanismes qui interviennent dans ce rejet et de tester différentes thérapies pour prévenir le rejet est d&#39;une importance critique. À cette fin, nous décrivons ici un modèle murin de greffe de la cornée qui a été en usage depuis plus de 20 ans pour étudier la greffe de cornée dans un environnement expérimental contrôlé. Comme les réponses de transplantation impliquent de nombreux facteurs différents qui travaillent de concert, qui sera ultime de déterminer si le tissu transplanté échoue ou réussisse, il est impossible de comprendre l&#39;importance de ces facteurs dans un modèle in vitro. Par conséquent, les études sur des animaux intacts sont nécessaires pour déterminer quels facteurs sont importants à succès ou failure du tissu transplanté. Alors que d&#39;autres espèces d&#39;animaux ont été utilisés pour étudier la greffe de cornée, le modèle murin a plusieurs avantages par rapport à l&#39;utilisation d&#39;autres espèces. Le premier est l&#39;existence de nombreuses souches de souris qui expriment certains transgènes ou ont été gène ciblées à l&#39;absence d&#39;expression de facteurs immunologiques spécifiques dont la fonction dans la transplantation peut être mieux étudié. En outre, il ya beaucoup de réactifs (deux facteurs recombinantes et d&#39;anticorps qui neutralisent facteurs) qui sont spécifiques pour les souris et qui ne existent pour de nombreuses autres espèces d&#39;animaux. En raison de l&#39;existence de ces facteurs, ce modèle a été largement utilisée pour identifier les facteurs impliqués dans la cornée allogreffe réponses aiguës 15, 17,18,20 -29. En outre, un grand nombre de facteurs impliqués dans la transplantation de la cornée sont également connus pour être fonctionnels dans la transplantation d&#39;autres tissus.

<table border="0" cellpadding="0" cellspacing="0" width="573">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:71px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Zeiss Surgical Microscope</td>
			<td style="width:71px;">Zeiss</td>
			<td style="width:119px;">Rebuilt</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">1 mL Syringe</td>
			<td>BD</td>
			<td>305122</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3 mL Syringe</td>
			<td>BD</td>
			<td>309657</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">10 mL Syringe</td>
			<td>BD</td>
			<td>309602</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Vannus Scissors</td>
			<td style="width:71px;">Stortz</td>
			<td style="width:119px;">E-3387</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">11-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">717939M</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 2.0mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7520</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 1.5 mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7510</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Tricaine Hydrochloride 0.5%</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 0065-0741-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Healon</td>
			<td style="width:71px;">Abbott</td>
			<td style="width:119px;">Healon OVD</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Forceps</td>
			<td style="width:71px;">FST</td>
			<td style="width:119px;">11251-20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">7-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td align="right" style="width:119px;">8065</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">2.5% Phenylephrine HCl</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 61314-342-02</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">1% Tropicamide</td>
			<td style="width:71px;">Bausch &#38; Lomb</td>
			<td style="width:119px;">NDC-24208-585-59</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hamilton Syringe</td>
			<td style="width:71px;">Hamilton</td>
			<td style="width:119px;">7654-01</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">33 gauge needle</td>
			<td style="width:71px;">Hamilton</td>
			<td align="right" style="width:119px;">90033</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Cell Strainer (100 &#956;m nylon)</td>
			<td style="width:71px;">BD Falcon</td>
			<td align="right" style="width:119px;">352360</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Hemocytometer</td>
			<td style="width:71px;">Cardinal Health</td>
			<td style="width:119px;">B3175</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trypan Blue</td>
			<td style="width:71px;">Sigma</td>
			<td style="width:119px;">T8154</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine corneal transplantation: a model to study the most common form of solid organ transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

Le modèle murin de greffe de la cornée a été utilisé pour plus de 20 ans à caractériser avec succès des mécanismes à la fois de l&#39;allogreffe de cornée rejet 19-23 et la cornée allogreffe acceptation 13, 15,16,18, 24-27. Ce modèle a été utilisé pour établir l&#39;importance de l&#39;expression de FasL dans la cornée allogreffe acceptation, par le fait que les animaux qui manquent de FasL sont pas en mesure d&#39;accepter les allogreffes de cornée 15. Il a également...

Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

Murin de transplantation de la cornée: Un modèle pour étudier la forme la plus courante de la transplantation d&#39;organes solides

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Orthotopique aortique transplantation: un modèle de rat pour étudier le développement de la vasculopathie chronique

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

Recherche

Médecine

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

Une méthode pour isoler l&#39;îlot murin et transplantation rénale sous-capsulaires

Since the early pioneering work of Ballinger and Reckard demonstrating that transplantation of islets of Langerhans into diabetic rodents could normalize ...

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Murin col de l&#39;utérus Cœur, la transplantation en utilisant une technique de modification de manchette

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

Cathétérisme cardiaque chez la souris pour mesurer le volume de pression Relation: Étudier les effets Bowditch

Animal models that mimic human cardiac disorders have been created to test potential therapeutic strategies. A key component to evaluating these ...

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

Un modèle de culture d'orgue endothéliale de porc cornée utilisant des boutons cornéens fendus

Experimental research on corneal endothelial cells is associated with several difficulties. Human donor corneas are scarce and rarely available for ...

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Développement d’un modèle expérimental non invasif assisté au laser de la perte de cellules endothéliales cornéennes

Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

Complications réduites après reconnexion artérielle dans un modèle de rat de transplantation orthotopique de foie

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

Auto-transplantation orthotopique de rein dans un modèle porcin utilisant la conservation d’organe de 24 heures et la télémétrie continue

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for ...

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Transplantation intramyocardique d’hydrogels injectables à chargement MSC après l’infarctus du myocarde dans un modèle murin

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Lésion d’ischémie-reperfusion d’organe en simulant des changements hémodynamiques dans le modèle de greffe de foie de rat

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...