Name: murine corneal transplantation: a model to study the most common form of solid organ transplantation
Uploaded: 2014-11-17T13:00:00
Description: Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

The authors would like to thank the many individuals who have worked on and perfected this technique and have been responsible for the generation of many manuscripts both in this lab and others. This work was supported by National Institutes of Health Grant EY12707 (PMS) and an unrestricted grant from Research to Prevent Blindness to Department of Ophthalmology.

नोट: इस प्रक्रिया में उपयोग किए गए सभी जानवरों विजन फॉर रिसर्च एसोसिएशन और नेत्र विज्ञान बयान नेत्र और दृष्टि अनुसंधान में पशुओं के उपयोग के लिए और साथ ही सेंट लुइस विश्वविद्यालय में पशु निरीक्षण समि?...

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यहाँ वर्णित प्रत्यारोपण की murine मॉडल कारकों सबसे अच्छा दोनों अस्वीकृति 15,17,18,20, 26-30 और कॉर्निया की स्वीकृति 21-25 के साथ जुड़े रहे हैं की भविष्य कहनेवाला है कि एक मॉडल में मानव कॉर्निया allograft अस्वीकृति क?...

प्रत्यारोपण मानव में प्रदर्शन किया प्रत्यारोपण के सबसे सफल और आम प्रकार से एक है. इस सर्जरी से किया जाता है कारण चोट, संक्रामक रोग 1, या गैर-संक्रामक कार्निया रोग 2 के अन्य रूपों का परिणाम होते हैं. अमेरिका के नेत्र बैंक एसोसिएशन से आंकड़े 46,000 से अधिक (: restoresight.org/eye_banks/eye_banks.html पर वेब साइट देखें) 2011 में प्रदर्शन किया गया है कि संकेत मिलता है. अपनी सफलता का एक संकेत एलोजेनिक कॉर्निया ग्राफ्ट के लिए एक वर्ष असफलता की दर 10 से 15% करने के लिए और सफलता 70% 3-8 से अधिक है 5 साल में सीमा होती है. कई अध्ययनों से पता चला है के रूप में, कॉर्निया allografts की सफलता सीधे आंख एक immunologically विशेषाधिकार प्राप्त साइट है कि इस तथ्य से संबंधित है. एक प्रतिरक्षा विशेषाधिकार साइट के रूप में कॉर्निया की स्थिति के लिए जिम्मेदार कारकों कि suppres कॉर्निया द्वारा उत्पादित कॉर्निया में दोनों रक्त और लसीका वाहिकाओं की कमी, प्रतिजन पेश कोशिकाओं के एक रिश्तेदार अभाव, कारकों में शामिलप्रतिरक्षा प्रेरक funtions 9-15 है, एमएचसी की कम अभिव्यक्ति 16 एंटीजन, और FasL 17-20 की अभिव्यक्ति. हालांकि, सफलता के लिए इन ग्राफ्ट predisposing इन कारकों के बावजूद, वे अस्वीकृति 3-7 से गुजरना है. नतीजतन, इस अस्वीकृति मध्यस्थता कि उन तंत्र को समझने के साथ ही अस्वीकृति को रोकने के लिए विभिन्न उपचारों के परीक्षण के महत्व का है. कि अंत करने के लिए, हम यहां एक नियंत्रित प्रयोगात्मक वातावरण में प्रत्यारोपण का अध्ययन करने के लिए 20 साल के लिए उपयोग में किया गया है कि प्रत्यारोपण के एक murine मॉडल का वर्णन. प्रत्यारोपण प्रतिक्रियाओं परम प्रत्यारोपित ऊतक विफल रहता है या सफल होता है या नहीं यह निर्धारित होगा कि संगीत कार्यक्रम में काम कर रहे कई विभिन्न कारकों को शामिल है, यह किसी भी इन विट्रो मॉडल में उन कारकों के महत्व को समझने के लिए संभव नहीं है. नतीजतन, अक्षत जानवरों का उपयोग अध्ययन सफलता या failu या तो महत्वपूर्ण हैं क्या कारकों का निर्धारण करने के लिए आवश्यक हैंप्रत्यारोपित ऊतक के पुन. जानवरों की अन्य प्रजातियों प्रत्यारोपण अध्ययन करने के लिए इस्तेमाल किया गया है जबकि अन्य प्रजातियों का उपयोग करने के लिए जब तुलना में, murine मॉडल के कई फायदे हैं. पहले कुछ transgenes व्यक्त या जिसका समारोह प्रत्यारोपण में बेहतर अध्ययन किया जा सकता विशिष्ट प्रतिरक्षा कारकों की अभिव्यक्ति की कमी के लिए जीन-लक्षित किया गया है कि चूहों के कई उपभेदों का अस्तित्व है. इसके अलावा, पशुओं के कई अन्य प्रजातियों के लिए मौजूद नहीं है चूहों के लिए विशिष्ट हैं और जो कि कई अभिकर्मकों (कारकों को बेअसर कि पुनः संयोजक कारकों और एंटीबॉडी दोनों) कर रहे हैं. इन कारकों के कारण अस्तित्व का, इस मॉडल तीव्र कॉर्निया allograft प्रतिक्रियाएं 15, 17,18,20 -29 में शामिल प्रासंगिक कारकों की पहचान करने के लिए बड़े पैमाने पर इस्तेमाल किया गया है. इसके अलावा, प्रत्यारोपण में शामिल कारकों में से कई को भी अन्य ऊतकों के प्रत्यारोपण में कार्यक्षम होने के लिए जाना जाता है.

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			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:71px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Zeiss Surgical Microscope</td>
			<td style="width:71px;">Zeiss</td>
			<td style="width:119px;">Rebuilt</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">1 mL Syringe</td>
			<td>BD</td>
			<td>305122</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3 mL Syringe</td>
			<td>BD</td>
			<td>309657</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">10 mL Syringe</td>
			<td>BD</td>
			<td>309602</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Vannus Scissors</td>
			<td style="width:71px;">Stortz</td>
			<td style="width:119px;">E-3387</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">11-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">717939M</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 2.0mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7520</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 1.5 mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7510</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Tricaine Hydrochloride 0.5%</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 0065-0741-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Healon</td>
			<td style="width:71px;">Abbott</td>
			<td style="width:119px;">Healon OVD</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Forceps</td>
			<td style="width:71px;">FST</td>
			<td style="width:119px;">11251-20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">7-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td align="right" style="width:119px;">8065</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">2.5% Phenylephrine HCl</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 61314-342-02</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">1% Tropicamide</td>
			<td style="width:71px;">Bausch &#38; Lomb</td>
			<td style="width:119px;">NDC-24208-585-59</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hamilton Syringe</td>
			<td style="width:71px;">Hamilton</td>
			<td style="width:119px;">7654-01</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">33 gauge needle</td>
			<td style="width:71px;">Hamilton</td>
			<td align="right" style="width:119px;">90033</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Cell Strainer (100 &#956;m nylon)</td>
			<td style="width:71px;">BD Falcon</td>
			<td align="right" style="width:119px;">352360</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Hemocytometer</td>
			<td style="width:71px;">Cardinal Health</td>
			<td style="width:119px;">B3175</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trypan Blue</td>
			<td style="width:71px;">Sigma</td>
			<td style="width:119px;">T8154</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine corneal transplantation: a model to study the most common form of solid organ transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

प्रत्यारोपण के murine मॉडल को सफलतापूर्वक कॉर्निया allograft अस्वीकृति 19-23 और कॉर्निया allograft स्वीकृति 13, 15,16,18, 24-27 दोनों के तंत्र को चिह्नित करने के लिए 20 साल के लिए इस्तेमाल किया गया है. इस मॉडल FasL कमी है कि जानव...

Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

Murine प्रत्यारोपण: ठोस अंग प्रत्यारोपण के सबसे आम रूप अध्ययन के लिए एक मॉडल

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Orthotopic महाधमनी ट्रांसप्लांटेशन एक चूहा जीर्ण Vasculopathy के विकास अध्ययन के लिए मॉडल:

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

Murine आइलेट अलगाव और Subcapsular गुर्दा प्रत्यारोपण के लिए एक विधि

Since the early pioneering work of Ballinger and Reckard demonstrating that transplantation of islets of Langerhans into diabetic rodents could normalize ...

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

एक संशोधित गिरफ्तार तकनीक का प्रयोग murine सरवाइकल हृदय प्रत्यारोपण मॉडल

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

Bowditch &#39;प्रभाव की जांच: दबाव माप उपाय रिश्ते को चूहे में कार्डिएक कैथीटेराइजेशन

Animal models that mimic human cardiac disorders have been created to test potential therapeutic strategies. A key component to evaluating these ...

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

एक Porcin Corneal Endothelial अंग संस्कृति मॉडल विभाजन Corneal बटन का उपयोग

Experimental research on corneal endothelial cells is associated with several difficulties. Human donor corneas are scarce and rarely available for ...

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

कॉर्नियल एंडोथेलियल सेल लॉस के एक Noninvasive, लेजर-असिस्टेड प्रायोगिक मॉडल का विकास

Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

ऑर्थोटोपिक लिवर प्रत्यारोपण के चूहे मॉडल में धमनी पुनर्संबंध के बाद कम जटिलताओं

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

24 घंटे अंग संरक्षण और निरंतर टेलीमेट्री का उपयोग करके पोर्सिन मॉडल में ऑर्थोटोपिक किडनी ऑटो-ट्रांसप्लांटेशन

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for ...

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

मुरीन मॉडल में मायोकार्डियल इंफार्क्शन के बाद एमएससी-लोडिंग इंजेक्टेबल हाइड्रोगेल्स का इंट्रामायोकार्डियल प्रत्यारोपण

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

चूहा लिवर प्रत्यारोपण मॉडल में हेमोडायनामिक परिवर्तनों का अनुकरण करके अंग इस्केमिया-रिफ्यूजन इंजरी

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...