Name: murine corneal transplantation: a model to study the most common form of solid organ transplantation
Uploaded: 2014-11-17T13:00:00
Description: Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

The authors would like to thank the many individuals who have worked on and perfected this technique and have been responsible for the generation of many manuscripts both in this lab and others. This work was supported by National Institutes of Health Grant EY12707 (PMS) and an unrestricted grant from Research to Prevent Blindness to Department of Ophthalmology.

참고 :이 절차에 사용 된 모든 동물 비전 연구 협회와 안과 문 안과 및 비전 연구에서 동물의 사용에 대한뿐만 아니라 세인트 루이스 대학의 동물 감독위원회에 의해 아래로 설정 한 지침에 따라 처리됩니다. 참고 : 모든 수술 장비 및 솔루션은 눈의 세균 감염을 제한하기 위해 수술 전에 소독한다. 그것은 동물이 과정에서 약간의 통증을 경험 수행하면서, 우리는 진통제를 사용하지 않는다는...

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여기에 설명 된 각막 이식의 쥐 모델 요인이 가장 모두 거부 15,17,18,20, 26 ~ 30 각막의 수용 21-25과 관련된 무엇을 예측하는 모델에서 각막 이식 거부 반응을 연구하기 위해 연구자 수 있습니다 동종 이식. 환자를 치료하거나 예방하거나 거부 (31)에 어느 국소 또는 전신적 스테로이드 치료를 부여하는 각막 이식, 달리이 모델은 일반적으로 치료의 부재에서 거부 동종 관련된 ?...

각막 이식은 인간에서 수행 이식의 가장 성공적이고 일반적인 유형 중 하나입니다. 이 수술을 행하는 이유는 부상 한 감염성 질환, 또는 비 - 감염성 질환 각막이 다른 형태의 결과이다. 미국의 안구 은행 협회 숫자는 46,000 이상이 (: restoresight.org/eye_banks/eye_banks.html에서 웹 사이트 참조) 2011 년에 수행 된 것을 나타냅니다. 성공의 표시는 동종 각막 이식 1 년 고장율 10-15%과 성공이 70 % 3-8 초과 5 년 범위 것입니다. 많은 연구가 도시 한 바와 같이, 각막 동종 이식의 성공은 직접 눈 면역 특권 사이트 것과 관련되어있다. 면역 특권 사이트와 각막 상태에 책임 요소는 suppres 각막 의해 생성 각막 모두 혈액 및 림프 혈관 부족, 항원 제시 세포의 상대적인 부재, 요소를 포함면역 이펙터 funtions에게 9-15이야, MHC의 낮은 발현은 16 항원 및 FasL의 17 ~ 20의 발현. 그러나 성공이 이식을 걸리기 이러한 요인에도 불구하고, 그들은 거절 3-7를 받아야 않습니다. 따라서,이 거절을 매개 이러한 메커니즘을 이해뿐만 아니라 거부를 방지하기 위해 다양한 치료법을 테스트하는 것은 매우 중요하다. 이를 위해, 우리는 여기에 통제 된 실험 환경에서 각막 이식을 연구하기 위해 20 년 이상 사용되었습니다 각막 이식의 쥐 모델을 설명합니다. 이식 응답은 궁극적가 이식 된 조직이 실패 또는 성공 여부를 확인할 수 콘서트에서 일하는 많은 다른 요소들을 포함하기 때문에, 어느 하나의 시험 관내 모델에서 이들 요소의 중요성을 이해하는 것은 불가능하다. 따라서, 그대로 동​​물을 사용하는 연구가 성공 또는 failu 중 하나에 중요한 요인이 무엇인지를 결정해야한다이식 조직의 재. 동물의 다른 종 각막 이식을 연구하는 데 사용되었지만, 다른 종을 사용하는 것에 비해, 뮤린 모델은 몇 가지 장점을 갖는다. 첫 번째는 특정 유전자를 발현 또는 기능 이식의 더 나은 연구 할 수있는 특정 면역 인자의 발현을 부족한 유전자를 대상으로 한 마우스의 여러 변종의 존재입니다. 또한, 동물의 많은 다른 종의 존재하지 않는 마우스에 대한 특정 많은 시약 (요인을 중화 재조합 요인과 항체 모두)이 있습니다. 때문에 이들 인자의 존재,이 모델은 급성 각막 동종 이식 반응 15 17,18,20 -29 관련된 관련 요소를 식별하는 데 광범위하게 사용되어왔다. 또한, 각막 이식과 관련된 많은 요인은 다른 조직 이식 기능적 것으로 알려져있다.

<table border="0" cellpadding="0" cellspacing="0" width="573">
	<tbody>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:71px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Zeiss Surgical Microscope</td>
			<td style="width:71px;">Zeiss</td>
			<td style="width:119px;">Rebuilt</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">1 mL Syringe</td>
			<td>BD</td>
			<td>305122</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3 mL Syringe</td>
			<td>BD</td>
			<td>309657</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">10 mL Syringe</td>
			<td>BD</td>
			<td>309602</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Vannus Scissors</td>
			<td style="width:71px;">Stortz</td>
			<td style="width:119px;">E-3387</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">11-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">717939M</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 2.0mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7520</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trephine 1.5 mm</td>
			<td style="width:71px;">Katena</td>
			<td style="width:119px;">K 2-7510</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Tricaine Hydrochloride 0.5%</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 0065-0741-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Healon</td>
			<td style="width:71px;">Abbott</td>
			<td style="width:119px;">Healon OVD</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Forceps</td>
			<td style="width:71px;">FST</td>
			<td style="width:119px;">11251-20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">7-0 Sutures</td>
			<td style="width:71px;">Alcon</td>
			<td align="right" style="width:119px;">8065</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">2.5% Phenylephrine HCl</td>
			<td style="width:71px;">Alcon</td>
			<td style="width:119px;">NDC 61314-342-02</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">1% Tropicamide</td>
			<td style="width:71px;">Bausch &#38; Lomb</td>
			<td style="width:119px;">NDC-24208-585-59</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Hamilton Syringe</td>
			<td style="width:71px;">Hamilton</td>
			<td style="width:119px;">7654-01</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">33 gauge needle</td>
			<td style="width:71px;">Hamilton</td>
			<td align="right" style="width:119px;">90033</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Cell Strainer (100 &#956;m nylon)</td>
			<td style="width:71px;">BD Falcon</td>
			<td align="right" style="width:119px;">352360</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">Hemocytometer</td>
			<td style="width:71px;">Cardinal Health</td>
			<td style="width:119px;">B3175</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Trypan Blue</td>
			<td style="width:71px;">Sigma</td>
			<td style="width:119px;">T8154</td>
			<td></td>
		</tr>
	</tbody>
</table>

murine corneal transplantation: a model to study the most common form of solid organ transplantation

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each year. While several animal models exist for this procedure and mice are the species that is most commonly used. The reasons for using mice are the relative cost of using this species, the existence of many genetically defined strains that allow for the study of immune responses, and the existence of an extensive array of reagents that can be used to further define responses in this species. This model has been used to define factors in the cornea that are responsible for the relative immune privilege status of this tissue that enables corneal allografts to survive acute rejection in the absence of immunosuppressive therapy. It has also been used to define those factors that are most important in rejection of such allografts. Consequently, much of what we know concerning mechanisms of both corneal allograft acceptance and rejection are due to studies using a murine model of corneal transplantation. In addition to describing a model for acute corneal allograft rejection, we also present for the first time a model of late-term corneal allograft rejection.

각막 이식의 쥐 모델을 성공적으로 각막 이식 거부 19-23 각막 이식 수용 13, 15, 16, 18, ​​24 ~ 27 두의 메커니즘을 특성화하기 위해 20 년 이상 사용되어왔다. 이 모델은 FasL의 결여 즉 동물에서 각막 이식에서 수용 FasL의 발현의 중요성을 설정하는 데 사용 된 각막 동종 이식 (15)을 받아 들일 수 없었다. 이는 또한 혈관 내피 성장 인자 수용체 1 모르 처리 크게 각막 이식 생존 <...

Watch this Scientific Journal Video about Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation at JoVE.com

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Mice have been used as a model for studying many forms of transplantation, including corneal transplantation. We describe in this report a murine model for both acute and late-term corneal transplantation.

쥐의 각막 이식 : 고체 장기 이식의 가장 일반적인 형태를 연구하기위한 모델

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Orthotopic 대동맥 이식 : 만성 Vasculopathy의 개발 연구에 생쥐 모델

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

연구

의학

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

Murine 아일릿 절연 및 Subcapsular 신장 이식을위한 방법

Since the early pioneering work of Ballinger and Reckard demonstrating that transplantation of islets of Langerhans into diabetic rodents could normalize ...

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

수정 커프 기술을 사용하여 쥐의 자궁 심장 이식 모델

Mouse models are of special interest in research since a wide variety of monoclonal antibodies and commercially defined inbred and knockout strains are ...

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

Bowditch 효과 조사 : 압력 볼륨 관계를 측정하기 위해 마우스의 심장 도관

Animal models that mimic human cardiac disorders have been created to test potential therapeutic strategies. A key component to evaluating these ...

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

분할 각막 단추를 사용하여 돼지 각막 내피 내피 기관 문화 모형

Experimental research on corneal endothelial cells is associated with several difficulties. Human donor corneas are scarce and rarely available for ...

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

각막 내피 세포 손실의 비 침습적, 레이저 보조 실험 모델의 개발

Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

직교 간 이식의 쥐 모형에 있는 동맥 재연결 후에 감소된 합병증

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry

24시간 장기 보존 및 지속적인 원격 분석을 사용하여 돼지 모델에서 직소 신장 자동 이식

In the present era of organ transplantation with critical organ shortage, various strategies are employed to expand the pool of available allografts for ...

Intramyocardial Transplantation of MSC-Loading Injectable Hydrogels after Myocardial Infarction in a Murine Model

Murine 모델에서 심근 경색 후 MSC 로딩 주사용 하이드로겔의 자궁 내 이식

One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

쥐 간 이식 모형에 있는 혈역학적인 변경을 시뮬레이션하여 기관 허혈-재관류 상해

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...