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DOI :
10.3791/51857-v
October 28th, 2014
Chapters
0:05
Title
1:23
Cell Culture and Calcium Dye Loading
2:36
Preparation of Cells for Flow Cytometry
3:28
Flow Cytometer Setup and Flow Cytometry Measurements
5:33
Data Analysis
7:57
Results: Changes in Cytosolic Calcium Concentration are Measured by Flow Cytometry
9:48
Conclusion
钙是参与许多生理学和病理生理学的信号通路。活细胞成像,需要专门的设备和非常耗时。使用流式细胞仪来确定在纳入悬浮贴壁的上皮细胞内钙的相对变化快速,简单的方法进行了优化。
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