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DOI :
10.3791/51882-v
•
9:57 min
December 16th, 2014
Chapters
0:05
Title
1:17
Reverse Transfection siRNA Screening
3:20
Imaging and Image Segmentation
5:21
Data Extraction
7:39
Results: Representative Raw Individual Cell Data Processing
9:27
Conclusion
呈现是一个灵活的信息学的工作流程使得荧光标记的细胞的复用的基于图像的分析。工作流量化细胞核和细胞质的标记,并计算这些室之间的标记易位。提供了用于在96孔格式使用siRNA和可靠的方法为标记物的检测通过间接免疫荧光细胞的扰动过程。
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