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11.8K Views
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08:31 min
September 16th, 2014
DOI :
10.3791/51895-v
Chapters
0:05
Title
1:29
Protein Tagging Site and Fluorophore Selection
3:04
Luminescence Resonance Energy Transfer (LRET) Setup
5:00
Data Analysis
6:58
Results: Representative LRET Measurements
7:57
Conclusion
Summary
我们在这里介绍了一种改进荧光共振能量转移(LRET)方法,在这里我们介绍的供体和受体荧光基团的站点之间的蛋白酶裂解位点。该变形使得我们能够获得来自感兴趣膜蛋白,使膜蛋白的无蛋白质纯化的研究中出现的具体LRET信号。
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