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DOI :
10.3791/51899-v
September 15th, 2014
Chapters
0:05
Title
1:32
Preparation of Microfluidic Chambers
3:26
Dissection of Neuronal Culture and Plating on Chambers
5:41
Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
7:43
Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
10:27
Conclusion
BDNF、神経栄養因子の軸索輸送は、いくつかのニューロン集団の生存と機能に重要である。いくつかの変性疾患は、軸索の構造と機能の破壊でマークされています。私たちは、一次ニューロンを用いたマイクロ流体チャンバ内のQD-BDNFのライブ人身売買を検査するために使用される技術を実証した。
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