Name: a radio-telemetric system to monitor cardiovascular function in rats with spinal cord transection and embryonic neural stem cell grafts
Uploaded: 2014-10-07T14:00:00
Description: Watch this Scientific Journal Video about A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts at JoVE.com

The work was supported by grants from NIH/NINDS (NS054883), Craig H. Neilsen Foundation (280072), and the Veterans Administration and Canadian Spinal Research Organization. We thank the Rat Resource and Research Center, University of Missouri, Columbia, Missouri, for providing GFP rats.

Alle dyr protokoller blev godkendt af Institutional Animal Care og brug Udvalg (IACUC). NIH retningslinjer for forsøgsdyr, pleje og sikkerhed er strengt følges. Dyr med kirurgiske procedurer er blevet tilstrækkeligt behandlet for at minimere smerte og ubehag. 1. rygmarv Curgery og Cell Podning <ol><li> Autoklave kirurgiske instrumenter forud for alle operationer. Brug Hot Bead Sterilizer (Fine Science Tools) til at fjerne patogener og mikrobielle forureninger fra instrumenter mellem pro...

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Traditionelt er en væskefyldt kanyle indsat i arterien og forbindes til en tryktransducer til at optage kardiovaskulære parametre som en terminal snapshot i hvert dyr 11. Til kontinuerligt at overvåge hjerte-kar ydeevne i lang tid, er radio-telemetri systemer anvendes i mange laboratorier. Denne mere raffineret værktøj kan optage blodtrykket i bevidst, frit bevægelige dyr. Sammenlignet med væskefyldte katetre er telemetri betragtes som en mere avanceret teknik til præcist at vurdere kardiovaskulær fu...

Kardiovaskulær dysfunktion opstår efter rygmarvsskade (SCI) på et højt niveau. Det manifesterer sig i uordnede blodtryk og puls i hvile, ortostatisk hypotension, anstrengelsesudløst hypotension og autonom dysrefleksi karakteriseret ved episoder af hypertension og baroreflex-medieret bradykardi som reaktion på sensoriske stimuli under skaden niveau 1,2. Disse symptomer forstyrre dagligdagen for rygmarvsskadede patienter. Derfor er det vigtigt at etablere effektive redskaber til undersøgelse af kardiovaskulære ændringer i dyr med SCI og eksperimentelle behandlinger. At undersøge kardiovaskulære funktion i dyr, er adskillige teknikker blevet anvendt til at overvåge blodtryk og hjertefrekvens. Centrale kardiovaskulære parametre kan registreres af kanyleindføringsdel og telemetri, mens invasive hale-manchetter kan anvendes til at måle perifert blodtryk 3. Sammenlignet med andre metoder, teleMetry har den største fordel, at det giver mulighed for kontinuerlig optagelse i frit bevægelige dyr og langsigtet overvågning af hjerte-kar-funktion 4. I SCI dyremodeller, kan ændringerne i perifert blodtryk efter eksperimentel stimulation ikke være stor nok til at blive opdaget. Derfor bør vælges passende hjertemonitorering teknik til dyr med SCI. I den foreliggende undersøgelse blev en radio-telemetri, der blev indført for at overvåge hjerte-kar-funktion hos voksne rotter efter komplet rygmarv transection. Rotter modtog transplantater af syngene rotte embryonal dag 14 (E14) hjernestamme-afledte neurale stamceller (BS-NSC) i læsionsstedet. Rotter med skade og ingen transplantation og naive, uskadt rotter tjente som kontroller. Proceduren telemetri omfatter senderen sterilisering og implantation (figur 1), registrering af basal kardiovaskulær parameter colorektale distension-inducerede reaktioner og senderen rengøring ogopbevaring.

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			<td colspan="2" height="21" style="height:21px;width:315px;">Table of Specific Materials/Equipment:</td>
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			<td height="41" rowspan="2" style="height:41px;width:120px;">Reagents</td>
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			<td height="83" rowspan="2" style="height:83px;width:120px;">Fibrinogen (rat)</td>
			<td rowspan="2" style="width:195px;">Sigma</td>
			<td rowspan="2" style="width:103px;">F6755-25MG</td>
			<td rowspan="2" style="width:164px;">2 hr at 37oC to dissovle</td>
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			<td height="80" rowspan="2" style="height:80px;width:120px;">Thrombin (rat)</td>
			<td rowspan="2" style="width:195px;">Sigma</td>
			<td rowspan="2" style="width:103px;">T5772-100UN</td>
			<td rowspan="2" style="width:164px;">Dissovle in 10 mM CaCl2</td>
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			<td height="42" style="height:42px;width:120px;">1% Terg-A-Zyme</td>
			<td style="width:195px;">Sigma</td>
			<td style="width:103px;">Z273287</td>
			<td style="width:164px;">Enzymatic solution for telemeter cleaning</td>
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			<td height="42" style="height:42px;width:120px;">Fluorogold</td>
			<td style="width:195px;">Fluorochrome</td>
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			<td style="width:164px;">Dissovle in distilled water and avoid light</td>
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			<td height="42" style="height:42px;width:120px;">Telemeter&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160; (PA-C40)</td>
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			<td height="105" style="height:105px;width:120px;">Telementric recording and analysis system</td>
			<td style="width:195px;">Data Sciences International</td>
			<td style="width:103px;"></td>
			<td style="width:164px;">Signal stimulator, Data Exchange Matrix, receivers, Ambient pressure reference monitor</td>
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		<tr height="63">
			<td height="63" style="height:63px;width:120px;">Balloon-tipped catheter</td>
			<td style="width:195px;">Edward Lifesciences</td>
			<td style="width:103px;">111F7-P</td>
			<td style="width:164px;">For colorectal distension</td>
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a radio-telemetric system to monitor cardiovascular function in rats with spinal cord transection and embryonic neural stem cell grafts

Høj thorax eller cervikal rygmarvsskade (SCI) kan føre til hjerte-kar-dysfunktion. Til overvågning kardiovaskulære parametre, vi implanteret et kateter forbundet til en radiosender i den femorale arterie i rotter, der undergik en T4 rygmarven overskæring med eller uden podning af embryonale hjernestamme-afledte neurale stamceller, der udtrykker grønt fluorescerende protein. Sammenlignet med andre metoder såsom kanyleindføringsdel eller hale-manchet, telemetri er fordelagtig til løbende at overvåge blodtryk og hjertefrekvens i frit bevægelige dyr. Det er også i stand til langsigtede flere opkøb data. I rygmarvsskadede rotter lykkedes registreret basale kardiovaskulære data i henhold til uhæmmet tilstand og autonom dysrefleksi som reaktion på kolorektal udspiling. Derudover kan kardiovaskulære parametre før og efter SCI sammenlignes i samme rotte, hvis en sender implanteres før rygmarv overskæring. En begrænsning af den beskrevne telemetRy procedure er, at implantation i den femorale arterie kan påvirke blodforsyningen til den ipsilaterale bagben.

Anvendelse af den ovenfor beskrevne telemetri teknik, vi med succes optaget kardiovaskulære parametre i rygmarvsskadede dyr. Hos dyr med SCI alene, blev det flerårige væsentligt reduceret hvorimod HR øget i forhold til naive dyr, i overensstemmelse med tidligere rapporter 9. Hos dyr med BS-NSC podning, MAP og HR nærmede niveauer, der måles i naive dyr (Figur 2). Under kolorektal udspiling blev en rotte betragtet som dysreflexic hvis skadelige stimulation produceret en stigning i MAP og ...

Watch this Scientific Journal Video about A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts at JoVE.com

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

Vi præsenterer en protokol for at bruge en radio-telemetrisk system til registrering kardiovaskulære parametre i T4 rygmarv overskårne rotter otte uger efter det embryonale hjernestammen neural stamcelle podning i læsionsstedet. Telemetri er en avanceret teknik til præcist at vurdere den kardiovaskulære funktion i bevidste frit bevægelige rygmarvsskadede rotter.

En radio-telemetri system til overvågning Kardiovaskulær funktion i rotter med Spinal Cord transection og embryonale neural stamcelle transplantater

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

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