Name: corneal donor tissue preparation for descemet's membrane endothelial keratoplasty
Uploaded: 2014-09-17T14:00:00
Description: Watch this Scientific Journal Video about Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty at JoVE.com

1，建立无菌区的<ol><li>唐合适的服装，包括帽子和口罩。戴防护眼镜，在处理室时。 </li><li>准备处理区域。当使用一个加工室，维持风扇的连续操作，以创建在室内的正压。 </li><li>收集所有用品。检查到期日期，包装完整性，灭菌指标。 </li><li>洗手，不要非无菌手套。 </li><li>打开无菌包，以建立一个无菌区。当使用一个加工室，放在桌上的包和无菌条件下打开包裹。不利用外部1.5英寸的卷体...

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	<li>Fernandez, M., Afshari, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+Keratoplasty:+From+DLEK+to+DMEK.">Endothelial Keratoplasty: From DLEK to DMEK.</a> Middle East African Journal of Ophthalmology. 17 (1), 5-8 (2010).</li><li>Kruse, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Stepwise+Approach+to+Donor+Preparation+and+Insertion+Increases+Safety+and+Outcome+of+Descemet+Membrane+Endothelial+Keratoplasty.">A Stepwise Approach to Donor Preparation and Insertion Increases Safety and Outcome of Descemet Membrane Endothelial Keratoplasty.</a> Cornea. 30 (5), 580-587 (2011).</li><li>Dapena, I., Moutsouris, K., Droutsas, K., Ham, L., van Dijk, K., Melles, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Standardized+'No-Touch'+Technique+for+Descemet+Membrane+Endothelial+Keratoplasty.">Standardized 'No-Touch' Technique for Descemet Membrane Endothelial Keratoplasty.</a> Archives of Ophthalmology. 129, 88-94 (2011).</li><li>Tourtas, T., Laaser, K., Bachmann, B., Cursiefen, C., Kruse, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Descemet+Membrane+Endothelial+Keratoplasty+versus+Descemet+Stripping+Automated+Endothelial+Keratoplasty.">Descemet Membrane Endothelial Keratoplasty versus Descemet Stripping Automated Endothelial Keratoplasty.</a> American Journal of Ophthalmology. 153 (6), 1082-1090 (2012).</li><li>Guerra, F., Anshu, A., Price, M., Price, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+Keratoplasty:+fellow+eyes+comparison+of+Descemet+stripping+automated+endothelial+keratoplasty+and+Descemet+membrane+endothelial+keratoplasty.">Endothelial Keratoplasty: fellow eyes comparison of Descemet stripping automated endothelial keratoplasty and Descemet membrane endothelial keratoplasty.</a> Cornea. 30 (12), 1382-1386 (2011).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> 2012 Eye Banking Statistical Report: Surgical Use and Indications for Corneal Transplant Statistical Report Analysis. , (2012).</li><li>Price, M., Price, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Descemet's+stripping+endothelial+keratoplasty.">Descemet's stripping endothelial keratoplasty.</a> Current Opinion Ophthalmology. 18 (4), 290-294 (2007).</li><li>Woodward, M., Titus, M., Mavin, K., Shtein, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Corneal+Donor+Tissue+Preparation+for+Endothelial+Keratoplasty.">Corneal Donor Tissue Preparation for Endothelial Keratoplasty.</a> Journal of Visualized Experiments. (64), e3847 (2012).</li><li>Price, M., Price, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Descemet's+membrane+endothelial+keratoplasty+surgery:+update+on+the+evidence+and+hurdles+to+acceptance.">Descemet's membrane endothelial keratoplasty surgery: update on the evidence and hurdles to acceptance.</a> Current Opinion Ophthalmology. 24 (4), 329-335 (2013).</li><li>Venzano, D., Pagani, P., Randazzo, N., Cabiddu, F., Traverrso, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Descemet+Membrane+air-bubble+separation+in+donor+cornea.">Descemet Membrane air-bubble separation in donor cornea.</a> Journal of Cataract and Refractive Surgery. 36 (12), 2022-2027 (2010).</li><li>Price, F., Price, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> DSEK: What you need to know about endothelial keratoplasty. , (2009).</li><li>Studeny, P., Sivekova, D., Lieheova, K., Vokrojova, M., Kuchynka, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hybrid+technique+of+lamellar+keratoplasty+(DMEK-S).">Hybrid technique of lamellar keratoplasty (DMEK-S).</a> Journal of Ophthalmology. 2013, 2013.254383 (2013).</li><li>Muraine, M., Gueudry, J., He, Z., Piselli, S., Lefevre, S., Toubeau, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+technique+for+the+preparation+of+corneal+grafts+for+Descemet+membrane+endothelial+keratoplasty.">Novel technique for the preparation of corneal grafts for Descemet membrane endothelial keratoplasty.</a> American Journal of Ophthalmology. 156 (5), 851-859 (2013).</li><li>Lie, J. T., Birbal, R., Ham, L., van der Wees, J., Melles, G. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Donor+tissue+preparation+for+Descemet+membrane+endothelial+keratoplasty.">Donor tissue preparation for Descemet membrane endothelial keratoplasty.</a> Journal of Cataract Refractive Surgery. 34 (9), 1578-1583 (2008).</li><li>Kruse, F. E., Laaser, K., Cursiefen, C., Heindl, L. M., Schlotzer-Schrehardt, U., Riss, S., Bachmann, B. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+stepwise+approach+to+donor+preparation+and+insertion+increases+safety+and+outcome+of+Descemet+membrane+endothelial+keratoplasty.">A stepwise approach to donor preparation and insertion increases safety and outcome of Descemet membrane endothelial keratoplasty.</a> Cornea. 30 (5), 580-587 (2011).</li><li>Rudolph, M., Kaaser, L., Bachmann, B., Cursiefen, C., Epstein, D., Kruse, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Corneal+higher-order+aberrations+after+Descemet&#8217;s+membrane+endothelial+keratoplasty.">Corneal higher-order aberrations after Descemet&#8217;s membrane endothelial keratoplasty.</a> Ophthalmology. 119 (3), 528-535 (2012).</li><li>Price, F., Price, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+endothelial+keratoplasty.">Evolution of endothelial keratoplasty.</a> Cornea. 32, 28-32 (2013).</li><li>Dapena, I., Dapena, L., Dirisamer, M., Ham, L., Melles, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visual+acuity+and+endothelial+cell+density+following+Descemet+membrane+endothelial+keratoplasty+(DMEK).">Visual acuity and endothelial cell density following Descemet membrane endothelial keratoplasty (DMEK).</a> Arch Soc Esp Oftalmol. 86 (12), 395-401 (2011).</li></ol>

角膜内皮细胞层起着通过脱水维持角膜透明度的关键作用。内皮细胞的疾病都可以导致显著视力障碍。 DMEK旨在与供体移植物，包括后弹力层和内皮细胞层取代病变血管内皮细胞。供体组织编制了一个完整的DM和足够的内皮细胞计数是移植组织的生存至关重要。糖尿病供体组织筹备过程中撕裂可导致组织损失和捐助组织的浪费。 DMEK的广泛应用已经在准备移植的组织和掌握复杂的手术技术所面临的?...

角膜内皮移植术（EK）已经迅速取代于2012年6穿透性角膜移植术（PKP）作为首选治疗内皮疾病占美国移植手术的50％以上的步骤。PKP是由长期的视觉康复高度散光的限制和屈光不稳定，全球诚信和缝线相关并发症的损失。初代EK程序，包括深板层内皮角膜（DLEK）参与角膜基质偏厚供体组织移植的人工清扫。这些程序成功地移植供体血管内皮组织，同时保持一个完整的角膜表面，但在技术上具有挑战性，由于不规则角膜板层接口，1导致有限的最佳矫正视力。选择目前EK程序后弹力层剥除内皮角膜移植术（DSEK），提供角膜CLAR性与更快的恢复视力，减少术后屈光度，散光，并降低外伤伤口裂开的风险，同时保持角膜表面7。此过程涉及去除病变后弹力膜和内皮细胞和置换部分的厚度供体角膜组织，包括一个新的内皮细胞层。供体角膜的解剖与角膜板层刀来准备后组织被移植8。由眼银行的供体组织的制备一直在快速增长，可以说，这个程序的成功。 2005年至2012年的近16倍的DSEK程序在美国对应目前正在编写的眼睛6银行组织的近一半。 虽然DSEK可以提高视力，以20/40以上，多数患者没有达到正常视力，由于间质混浊的界面和增强的光从另外捐助基质组织7的散落一地。该EK手术技术已经发展到后弹力膜内皮角膜移植术（DMEK）涉及的只给予体后弹力膜（DM）和血管内皮细胞的移植。本程序有谁达到正常视力，视力恢复快，而血管内皮抑制9的风险较低的患者比例较高。然而，准备供体组织是更具挑战性的是由于移植物更薄的尺寸。

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			<td height="42" style="height:42px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:268px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:167px;">Comments/Description</td>
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			<td height="21" style="height:21px;width:216px;">VisionBlue&#160;</td>
			<td style="width:268px;">D.O.R.C., Zuiland, The Netherlands</td>
			<td style="width:119px;">NA</td>
			<td></td>
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			<td height="21" style="height:21px;width:216px;">Barron vacuum punch</td>
			<td style="width:268px;">Katena, Denville, New Jersey</td>
			<td style="width:119px;">K20-2112</td>
			<td></td>
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corneal donor tissue preparation for descemet's membrane endothelial keratoplasty

后弹力膜内皮角膜移植术（DMEK）是角膜移植，其中只有一个单细胞层，角膜内皮细胞，连同其基底膜（Descemet膜）被引入到接收方的后基质3的形式。与角膜后弹力层剥除自动内皮角膜移植术（DSAEK），其中更多的捐助基质的介绍，不自然基质对基质的界面创建。其结果，角膜的天然解剖学保留尽可能允许改善恢复时间和视力4。角膜内皮移植术（EK）是首选的治疗内皮功能障碍的过程。 EK的优点包括快速恢复视力，保护眼部的完整性和最小的屈光变化，由于采用了小型，外围切口1。 DSAEK利用制备偏厚的基质和内皮细胞供体组织。快速SUCCESS和利用这个程序可以归因于眼睛银行可用性制备预切割的组织。眼库准备供体组织的好处包括：消除需要专门的设备备份供体组织的情况下，组织穿孔制备过程中的手术室和可用性。此外，高体积制备由眼库的技术人员供体组织可以提供供体组织的改进的质量。 DSAEK可能限制最佳矫正视力，由于创作的供体和受体角膜之间的间质界面。消除这个接口仅为捐助后弹力膜和内皮DMEK移植可以改善视觉效果和EK 5后减少并发症的发生。类似DSAEK，长期成功和验收DMEK的是依赖于易于预切割的可用性，眼库制备供体组织。在这里，我们提出了一个阶梯式的供体组织编写这可能会降低一些巴里ERS眼银行面临提供DMEK移植。

从基质适当分离，DM和内皮正在准备预切组织的DMEK必不可少的。五十捐献角膜为65±6岁（范围55-75），死亡2,616±321个/ mm 2至5.9±2.3天（范围1-10）和内皮细胞密度的处理时间（ECD）（平均年龄范围2,049-3,247）被用于由两个眼库的技术人员来评估手术制备技术的可行性和可重复性。成功率为72％，前25例，上升至80％，下半年76％的成功率。点二列相关（RPB）来确定，术前组织因子的相关性。捐?...

Watch this Scientific Journal Video about Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty at JoVE.com

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty

角膜供体组织准备后弹力层内皮角膜移植术

角膜内皮移植术是一种外科技术不断发展的进步导致的移植物薄，每个继承1移植。在这里，我们提出了一个技术控制的手动组织剥离，使血管内皮和后弹力捐助角巩膜按键膜移植2的安全和可重复的分离。

Descemet&#8217;s Membrane Endothelial Keratoplasty (DMEK) is a form of corneal transplantation in which only a single cell layer, the corneal endothelium, ...

Research

JoVE Journal

Medicine

Single Port Donor Nephrectomy

单端口捐赠者肾切除术

In 2007, Rane presented the first single port nephrectomy for a small non-functioning kidney at the World Congress of Endourology.  Since that time, the ...

科研

医学

Corneal Donor Tissue Preparation for Endothelial Keratoplasty

供体角膜内皮角膜移植术的组织准备

Over the past ten years, corneal transplantation surgical techniques have undergone revolutionary changes1,2. Since its inception, traditional full ...

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

制备和<em&gt;体外</em&gt;磁化的表征的miR-改性内皮细胞

To date, the available surgical and pharmacological treatments for cardiovascular diseases (CVD) are limited and often palliative. At the same time, gene ...

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

创面愈合研究的前活体角膜器官培养模型

The cornea has been used extensively as a model system to study wound healing. The ability to generate and utilize primary mammalian cells in two ...

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

使用分裂的角质按钮的孔膜内皮器官培养模型

Experimental research on corneal endothelial cells is associated with several difficulties. Human donor corneas are scarce and rarely available for ...

Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

角膜内皮细胞损失非侵入性激光辅助实验模型的发展

Nd:YAG lasers have been used to perform noninvasive intraocular surgery, such as capsulotomy for several decades now. The incisive effect relies on the ...

Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion

啮齿动物心脏和脑组织准备缺血后数码微距摄影

Macro photography is applicable for imaging various tissue samples at high magnification to perform qualitative and quantitative analyses. Tissue ...

An Epithelial Abrasion Model for Studying Corneal Wound Healing

研究角膜伤口愈合的上皮擦伤模型

The cornea is critical for vision, accounting for about two-thirds of the refractive power of the eye. Crucial to the role of the cornea in vision is its ...

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

一种猪异位心脏移植方案，用于向心脏同种异体移植物提供治疗药物

Cardiac transplantation is the gold standard treatment for end-stage heart failure. However, it remains limited by the number of available donor hearts ...

A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1

一种由工程成纤维细胞生长因子1刺激的Descemet剥离的人类角膜器官培养模型，仅具有加速愈合

Fuchs Endothelial Corneal Dystrophy (FECD) results from dysfunctional corneal endothelial cells (CECs) and is currently treated by transplantation of the ...