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DOI :
10.3791/51926-v
November 8th, 2014
Chapters
0:05
Title
2:03
Tissue Acquisition and Grossing
4:22
Maintenance of Established Organoid/Epithelial Cell Colonies
6:43
Results: Representative Mammosphere Imaging
8:51
Conclusion
使用完整的组织组织体原代细胞培养提供了一个模型系统,模仿的多细胞在体内微环境。我们开发了一种无血清乳腺原发上皮细胞组织培养模式,延续了混合细胞培养系,并表现出分化的形态,没有酶的组织破坏。乳房组织体保持活力> 6个月。
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