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DOI :
10.3791/51937-v
November 29th, 2014
Chapters
0:05
Title
1:25
Protein Sample and Buffer Preparation
2:38
Sensor Chip and SPR Instrument Setup
3:58
Experiment Cycles
7:32
Results: On Line Monitoring of Ligand Immobilization and Determination of Kinetic Rate Constants by Injections of Multiple Analyte Concentrations
9:07
Conclusion
表面プラズモン共鳴(SPR)は、リアルタイムでの生体分子相互作用を検出するためのラベルフリー法である。ここで、膜タンパク質のためのプロトコル:技術の長所と短所を議論しながら、受容体相互作用の実験は、記載されている。
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