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DOI :
10.3791/51942-v
October 19th, 2014
Chapters
0:05
Title
1:52
Human Tissue Samples and Cell Purification
4:14
Two-dimensional Electrophoresis
6:38
Cytoskeletal Activity Assays
8:17
Results: Identification of Prognostic Markers and Therapeutic Targets by 2DE and MS
9:50
Conclusion
ここでは、カップル2プロテオミクス技術、すなわち2次元電気泳動(2DE)および質量分析(MS)は、一次サンプルの2つ以上のグループ間で差次的に発現/翻訳後修飾タンパク質を同定するためのプロトコールを記載する。このアプローチは、一緒に機能的な実験と、予後マーカー/治療標的の同定および特徴を可能にします。
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