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DOI :
10.3791/51943-v
November 13th, 2014
Chapters
0:05
Title
1:35
Amplification of Plasmid Inserts and Addition of Poly T-tail by PCR
2:48
In Vitro Transcription
3:59
Treatment of Purified mRNA with Antartic Phosphatase
5:17
mRNA Transfection of Cells
7:01
Flow Cytometric Analyses of eGFP Expression in Cells
8:14
Results: Modified mRNA is Expressed in Human Cells
9:34
Conclusion
In diesem Video-Artikel beschreiben wir die in vitro Synthese von modifizierten mRNA zur Induktion der Proteinexpression in den Zellen.
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