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DOI :
10.3791/51953-v
September 25th, 2014
Chapters
0:05
Title
1:52
Cell Culture and Whole Virus Extraction
3:56
Viral Replication
4:55
RNA Extraction and RT-PCR
7:45
Confocal Microscopy
9:55
Results: Quantification of Cell Survivability in HoCV-01 Infected Cells
12:03
Conclusion
在这里,我们提出了一个协议来传播Homalodisca vitripennis细胞和HoCV-1 体外 。培养基从HoCV-1阳性培养去除和RNA的提取,每24小时为168小时。细胞生存力进行定量通过台盼蓝染色法。整个病毒粒子中提取的后感染。提取的RNA进行定量通过qRT-PCR。
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