Name: monitoring of systemic and hepatic hemodynamic parameters in mice
Uploaded: 2014-10-04T14:00:00
Description: Watch this Scientific Journal Video about Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice at JoVE.com

这项研究是由德国联邦Ministery教育与研究部（BMBF）资助的“虚拟肝络”的支持。我要感谢弗兰克·舒伯特和刘若英贡佩尔特从耶拿大学医院为他们生产的视频和创建动画和伊莎贝尔JANK录制音频帮助媒体中心。

住房和进行的所有程序均按照德国动物福利立法。 1，传感器校准（按照制造商的说明进行传感器校准） 1.1）米勒导管校准。预浸泡在导管的尖端在无菌水或盐水的前30分钟，以平衡（归零）和校准。 <ol><li>在米勒传感器连接到桥式放大器的millar1通道，插入米勒传感器尖端插入水柱。 </li><li>设置水柱值：0水柱2 O在数据分析软件窗口中，选择桥放...

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肝脏血流动力学监测是一项重要的研究工具，在肝病和肝胆外科。收购肝血流动力学数据有助于表征循环系统肝胆程序的影响。还需要采集的肝血流动力学数据，研究了影响门脉压力和流量的门户， 如药物的疗效，需要在研究评估血管活性药物。 尽管小尺寸的重要参数，系统性和肝血流动力学可以在小鼠中进行监测。分别在协议中的关键步骤如下：首先，以放置在一?...

这部影片的总体目标是要证明一个实时监控程序，用于获取系统和肝脏血流动力学参数。开发这个程序的基本原理是在需要获得系统和肝脏血流动力学参数老鼠实验干预其巨大的价值。该过程可以应用于幼稚动物和期间或之后给定的肝胆实验外科干预，如肝部分切除，门静脉结扎和肝脏移植。 收购肝血流动力学数据，啮齿动物，需要提出的侵入性手术。肝脏灌注不能获得非侵入性。不过，也有收购全身血压的替代品。如尾袖套法8监测技术已被用于获取在大鼠和小鼠的血压。尾袖套法可以在有良知施加项动物。当测量血压时，动物需要被放置并固定在一个特定的不舒服的位置。在尾套设备的说明书，厂商指出，老鼠可能会变得紧张，并强调这可能削弱流通的尾巴。该种情况下，在尾巴获得的外周血压力可以比中央的血压低得多。 使用一系列传感器进行数据采集的集成多通道监测器进行全程监控程序。通过将导管插入后，在显微镜下仔细显微解剖和曝光的各容器中得到的血压。流速通过将跨音速流动探头每个容器周围测定。 我们已经报道了类似的术中监测程序导致了全面的一系列生理血流动力学数据堪比SINGL的老鼠来自其他团体7日报道E数据。因此，我们认为这个过程代表了良好的基础，使之适应鼠标，一个物种的10倍，比老鼠更小。在关键的区别与大鼠程序是利用Millar的导管，用于获取血压数据，而不是基于流体的导管系统。流动数据也获得了跨声速流动探头，只是很多小的比相应的大鼠血管。 由于动物的体积小，小鼠仪器在技术上是有挑战性的，但可行的。一旦仪器完成时，数据采集与主寿命数据分析是简单的，因为可以使用预定义的设置文件。设置文件，必须在一系列的实验中，开始一次定义，并且可以被存储并用于所有后续实验。 到现在为止，我们应用这个程序，以评估急性肝的实验血流动力学的影响。我们测得的CAP和PVP之前和紧接之后的70％部分肝切除（PH）和在夹紧/去夹紧的实验。我们夹在右叶表示质量接着位数的简短（5分钟）的夹紧和左外叶肝较完全的90％肝脏质量的20％的肝十二指肠韧带。去夹紧开始释放，从肝右叶后释放的中位数和左外叶夹。最大合模时间低于10分钟。

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monitoring of systemic and hepatic hemodynamic parameters in mice

在实验研究中使用的小鼠模型是对肝脏的生理和病理障碍的研究有着重要意义。然而，由于鼠标的体积小，适合于鼠标术监测过程的技术细节很少说明。此前我们已经报道了监控程序，以获取血流动力学参数的影响。现在，我们适应的程序来获取小鼠全身及肝脏血流动力学参数，一个物种的10倍，比老鼠更小。该薄膜显示出动物的仪表以及评估在小鼠中的全身和肝血流动力学所需要的数据采集过程。重要的参数，包括体温，呼吸率和心脏速率，记录整个过程。全身血流动力学参数，包括颈动脉压（CAP）和中心静脉压（CVP）的。肝脏灌注参数包括门户vEIN压力（PVP），门脉流量以及肝总动脉（表1）的流量。仪器仪表和数据采集，记录的正常值是在1.5小时内完成。在此过程中的全身和肝血流动力学参数保持在正常范围内。 这个过程是具有挑战性的，但可行的。我们已经应用这个程序，以评估在正常小鼠以及在70％部分肝切除和肝瓣合模实验肝脏血流动力学。意思是PVP切除后（N = 20），为11.41±2.94 水柱这是显著高于对照组（P &lt;0.05）切除术（6.87±2.39 水柱 ）之前。肝叶夹紧实验的结果表明，该监视过程是敏感的，并适合于检测在入口压力和入口流速的微小变化。总之，这个过程是可靠的一个有经验的微型外科医生的手，但应限制为experiments地方是绝对需要的小鼠。

小鼠的重要参数，例如呼吸速率和心脏速率明显高于对大鼠高得多。平均全身血压和颈静脉压力类似于鼠值甚至类似于人的数据。 肝脏血流动力学的数据有明显的不同。我们从8只获得正常的值。在正常小鼠门脉血流之间的1.6〜2.3毫升/分钟之间。流入肝总动脉介于0.10至0.35毫升/分钟。在正常动物门静脉压力在大范围从4.4到11.2 水柱与8.09±2.47的平均值水柱 （表1...

Watch this Scientific Journal Video about Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice at JoVE.com

Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice

这部影片展示了如何获取小鼠全身及肝脏血流动力学。整个监视包括采集的重要参数，全身血压，中心静脉压，肝总动脉流量，和门静脉压，以及门户网站流量小鼠。

全身及肝脏血流动力学参数的监测小鼠

The use of mouse models in experimental research is of enormous importance for the study of hepatic physiology and pathophysiological disturbances. ...

Research

JoVE Journal

Medicine

Assessing Changes in Volatile General Anesthetic Sensitivity of Mice after Local or Systemic Pharmacological Intervention

局部或全身药物干预后评估的变化小鼠中挥发性全身麻醉药敏感性

One desirable endpoint of general anesthesia is  the state of unconsciousness, also known as hypnosis. Defining the hypnotic state in animals is  less ...

科研

医学

Clinical Assessment of Spatiotemporal Gait Parameters in Patients and Older Adults

时空步态参数的患者和老年人的临床评估

Spatial and temporal characteristics of human walking are frequently evaluated to identify possible gait impairments, mainly in orthopedic and ...

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

系统性红斑狼疮的BM12诱导模型（SLE）的C57BL / 6小鼠

Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical and immunological manifestations. Several spontaneous and inducible ...

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

优化分析<em&gt;在体内</em&gt;和<em&gt;体外</em&gt;脂肪肝

Establishing a system of procedures to qualitatively and quantitatively characterize in vivo and in vitro hepatic steatosis is important for metabolic ...

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

通过实时血液动力学监测在小鼠中通过肝脏浸润建立的不受控制的出血性休克

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via ...

Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats

肝硬化大鼠门脉高压综合征的侵袭性血流动力学特征

This is a detailed protocol describing invasive hemodynamic measurements in cirrhotic rats for the characterization of portal hypertensive syndrome. ...

Invasive Hemodynamic Monitoring of Aortic and Pulmonary Artery Hemodynamics in a Large Animal Model of ARDS

ards 大型动物模型主动脉和肺动脉血流动力学的侵袭血流动力学监测

One of the leading causes of morbidity and mortality in patients with heart failure is right ventricular (RV) dysfunction, especially if it is due to ...

Measurement of the Hepatic Venous Pressure Gradient and Transjugular Liver Biopsy

肝静脉压梯度和转性肝活检测量

Here we provide a detailed protocol describing the clinical procedure of hepatic venous pressure gradient (HVPG) measurement in patients with advanced ...

Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs

由脑氧测量和猪期扩展血液动力学监测指导的标准化出血性休克诱导

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an ...

Invasive Hemodynamic Assessment for the Right Ventricular System and Hypoxia-Induced Pulmonary Arterial Hypertension in Mice

小鼠右心室系统及缺氧诱发肺动脉高血压的侵入性血液动力学评估

Pulmonary arterial hypertension (PAH) is a chronic and severe cardiopulmonary disorder. Mice are a popular animal model used to mimic this disease. ...