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45.1K Views
•
07:54 min
September 2nd, 2014
DOI :
10.3791/51960-v
Chapters
0:05
Title
1:20
Predifferentiation of THP-1 Macrophage and Preparation for Nucleofection
3:22
Nucleofection of THP-1 Macrophage
4:53
Post-Nucleofection Care
6:15
Gross Characterization of siRNA Transfection THP-1 Macrophages
7:21
Conclusion
Summary
このプロトコルは、高い細胞活力と分化と偏光のための完全なマクロファージの容量を維持しつつ、高いトランスフェクション効率でエレクトロポレーションによってsiRNAまたはプラスミドDNAを用いたヒトTHP-1マクロファージをトランスフェクションするための効率的で信頼性の高い手法を提案する。
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