Name: non-terminal blood sampling techniques in guinea pigs
Uploaded: 2014-10-11T14:00:00
Description: Watch this Scientific Journal Video about Non-Terminal Blood Sampling Techniques in Guinea Pigs at JoVE.com

我们承认安妮B.斯​​腾森，伊丽莎白五安徒生，贝琳达二Bringtoft和动物管理员在实验医学，腓特烈校园，健康与医疗科学学院，丹麦哥本哈根大学的系的娴熟援助。雅各Lønholdt是感谢在拍摄过程他出色的技术援助。本研究是在部分地由LIFEPHARM中心体内药理学与哥本哈根大学的支持。

所显示的程序，是根据食品，农业和渔业部根据批准的丹麦动物实验督察协议执行。 1，采血从侧隐或跗骨静脉<ol><li>执行此过程无需麻醉和一名调查员，人们处理的豚鼠，一个采集样品的帮助。 </li><li>剃后腿跗区域与一个电动剃须刀，直到该脉是可见的。确保该区域足够大，以避免毛皮与穿刺部位干涉。 </li><li>放置在温水中洗澡的后腿轻轻揉搓小腿，使血管更加扩张和明...

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K., Halliwell, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+fat+diets+and+pathology+in+the+guinea+pig+Atherosclerosis+or+liver+damage.">High fat diets and pathology in the guinea pig Atherosclerosis or liver damage.</a> Biochim Biophys Acta. 1832 (2), 355-364 (2013).</li><li>Tveden-Nyborg, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maternal+vitamin+C+deficiency+during+pregnancy+persistently+impairs+hippocampal+neurogenesis+in+offspring+of+guinea+pigs.">Maternal vitamin C deficiency during pregnancy persistently impairs hippocampal neurogenesis in offspring of guinea pigs.</a> PLoS One. 7 (10), 12-18391 (2012).</li><li>Schjoldager, J. G., Tveden-Nyborg, P., Lykkesfeldt, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prolonged+maternal+vitamin+C+deficiency+overrides+preferential+fetal+ascorbate+transport+but+does+not+influence+perinatal+survival+in+guinea+pigs.">Prolonged maternal vitamin C deficiency overrides preferential fetal ascorbate transport but does not influence perinatal survival in guinea pigs.</a> Br J Nutr. 110 (9), 1573-1579 (2013).</li><li>Greulich, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Secretory+products+of+guinea+pig+epicardial+fat+induce+insulin+resistance+and+impair+primary+adult+rat+cardiomyocyte+function.">Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function.</a> J Cell Mol Med. 15 (11), 2399-2410 (2011).</li><li>Swifka, J., Weiss, J., Addicks, K., Eckel, J., Rosen, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epicardial+fat+from+guinea+pig+a+model+to+study+the+paracrine+network+of+interactions+between+epicardial+fat+and+myocardium.">Epicardial fat from guinea pig a model to study the paracrine network of interactions between epicardial fat and myocardium.</a> Cardiovasc Drugs Ther. 22 (2), 107-114 (2008).</li><li>Pilny, A. 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相比于小鼠和大鼠，豚鼠是可怕的性质，必须将球接近并为了减少应力正确处理。但从动物福利的角度，谨慎行事，自然是一个优先事项;然而，这也将减少对所收集的数据，例如焦虑相关的效应的风险。增加血液中糖皮质激素的水平和葡萄糖12。同样，每天处理会，观察者的动物，以正常的程序，并且可以用于进一步降低应力。在一个特定的血液采样技术的实用技能应该先观察有?...

豚鼠是一种宝贵而有效的实验动物模型，由于一些生物相似于人类如维生素C，相当于血浆脂蛋白代谢酶和脂蛋白的配置文件，以及共享的相似性与人类胎盘和膳食供应的要求产前发展1-3,7,8。这使豚鼠一个有吸引力的和合适的模型用于研究饮食有关的疾病，如非酒精性脂肪肝疾病，心血管疾病和维生素C缺乏推定的效果，而且还例如效应的影响。上显影产妇饮食干预后代。 反复采血，常常需要在长期和短期的体内研究。由于种属特异性的解剖结构差异的抽样技术的调制是必要的，以便获取在豚鼠的血液样本相比其他啮齿类动物模型<sup&gt; 4,5。这包括变异在静脉和动脉的过程中，也即豚鼠没有尾巴，因而具有不同的要求和选择两个采样和处理。从隐或跗静脉取样，引起最小的不适和压力中的动物，并且不需要麻醉3,9。这种技术允许重复的少量血液（100-400微升）， 如样品。来确定血浆中的生化标志物。如果甚至更小的样本大小是足够的（50-100微升），这可以很容易地通过穿孔的耳静脉取。这种技术也可以进行有意识的动物，测定血糖10,11时是有用的，例如。从颈静脉取样允许收集更大量的血液（1-2毫升），但需要麻醉。 在这里，我们提出了四种不同的采血技术无论是有意识或麻醉的豚鼠。在procedures均为非终端程序提供的样品体积和样品的数量不超过在实验室动物6采血准则。正是在良好的科学的利益和动物福利，压力应保持在最低限度。因此，只有训练有素和有能力的个人应当履行动物的程序。

<table border="0" cellpadding="0" cellspacing="0" width="944">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Name of Material/ Equipment</td>
			<td style="width:295px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:315px;">Comments/Description</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Electric shaver</td>
			<td style="width:295px;">Wella (Contura), Denmark</td>
			<td style="width:119px;">273207</td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Syringes&#160;</td>
			<td style="width:295px;">B.Braun-Omnifix-F, Germany</td>
			<td style="width:119px;">9161406V</td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Needles</td>
			<td style="width:295px;">BD Microlance,Spain</td>
			<td style="width:119px;">301156</td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Microvette collection tube&#160;</td>
			<td style="width:295px;">Sarstedt, D-51588N&#252;mbrecht, Germany</td>
			<td style="width:119px;">201,288,100</td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Accu-Check analyzer</td>
			<td style="width:295px;">Roche A/S Diagnostics, Denmark</td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Heparin 5000 IE/ml</td>
			<td style="width:295px;">LEO Pharma, Denmark</td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Anesthesia</td>
			<td style="width:295px;">Zoletil 50, Chemvet, Denmark</td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Anesthesia</td>
			<td style="width:295px;">Narcoxyl vet 20 mg/ml, Intervet, Denmark</td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Anesthesia</td>
			<td style="width:295px;">Torbugesic vet 10 mg/ml, Scanvet, Denmark</td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:216px;">Neutral eye ointment</td>
			<td style="width:295px;"></td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Gauze or a cotton swab&#160;</td>
			<td style="width:295px;"></td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Disinfectant (70% alcohol)</td>
			<td style="width:295px;"></td>
			<td style="width:119px;"></td>
			<td>Alternative product may be used. Multiple suppliers</td>
		</tr>
	</tbody>
</table>

non-terminal blood sampling techniques in guinea pigs

豚鼠拥有多种生物相似性和人类进行验证实验动物模型1-3。然而，使用豚鼠的目前代表的研究和描述性数据相对窄的区域上的特定方法是相应稀少。豚鼠的解剖学特征是从其他啮齿类动物模型略有不同，因此抽样技术调制，以适应物种特异性差异， 如 ，相对于小鼠和大鼠中，有必要获得足够的和高质量的样本。由于长期和短期的体内研究往往需要反复采血技术的选择，应很好地考虑，以减少动物的紧张和不适，也能保证生存，以及符合样本容量和可访问性的要求。静脉血液样本可在多个豚鼠如网而得到。中，隐和颈脉，每片含有利有弊技术4,5。在这里，我们提出了四种不同的采血技术无论是有意识或麻醉的豚鼠。该程序是所有的非终端程序提供的样品体积和样品的数量不超过在实验室动物6采血准则。所有描述的方法已经被彻底的测试和应用重复体内血液取样研究在我们的研究设施。

四种不同的方法来在任一有意识或麻醉的豚鼠非末端体内采血技术已经提出了图1示出了在豚鼠和可能的穿刺部位的横向隐和跗静脉的过程。耳静脉上的豚鼠耳的背侧表面可见被示于图2中这些静脉，可用于血液采样时只需要非常小的体积。豚鼠的颈静脉采血的位置显示在图3。颈静脉的课程由按建议的穿刺点最上面的箭头指向的箭头所示。 <p class="jove...

Watch this Scientific Journal Video about Non-Terminal Blood Sampling Techniques in Guinea Pigs at JoVE.com

Non-Terminal Blood Sampling Techniques in Guinea Pigs

豚鼠非终端采血技术

虽然已知的模型，豚鼠目前代表在实验动物科学的细分市场，有限的数据可在大多数程序的执行。在这里，我们介绍四种不同的方式在体内血液采样技术的非终端无论是有意识或麻醉的豚鼠。

Guinea pigs possess several biological similarities to humans and are validated experimental animal models1-3. However, the use of guinea pigs currently ...

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The enteric nervous system (ENS) is a self-contained network with identified functions, capable of performing complex behaviors in isolation. Its neurons ...

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Fluorescence microscopy of cells loaded with fluorescent, Ca2+-sensitive dyes is used for measurement of spatial and temporal aspects of Ca2+ signaling in ...

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Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help ...

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The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since ...

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Blood samples are commonly obtained in many experimental contexts to measure targets of interest, including hormones, immune factors, growth factors, ...

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Repeated blood collection is one of the most common techniques performed on laboratory animals. However, a non-lethal protocol for blood collection from ...

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基于 X 射线 Microcomputed 层析成像的玉米血管束微米尺度分型技术研究

It is necessary to accurately quantify the anatomical structures of maize materials based on high-throughput image analysis techniques. Here, we provide a ...