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DOI :
10.3791/51984-v
December 7th, 2014
Chapters
0:05
Title
1:33
Preparation of Random Mutation Library by Random Mutagenisis
3:38
Production of Retroviral Supernatants
5:13
Selection of Resistant Clones In Vitro
8:15
In Vitro Validation of Resistant Mutations
9:58
Results: Identification and Validation of Ruxolitinib Resistant Mutations
12:04
Concluion
キナーゼ阻害剤治療に対する遺伝的抵抗性の出現は、効果的な癌治療のための重要な課題を提起する。新たに開発された薬剤に対して耐性変異の同定および特徴は、より良い臨床管理、次世代薬剤設計に役立ちます。ここでは、in vitroスクリーニングおよび耐性変異の検証のために私たちのプロトコルを記述します。
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