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DOI :
10.3791/51991-v
September 24th, 2014
Chapters
0:05
Title
1:48
Chick Dissection and Isolation of DRGs
4:54
Dissociation, Enriching, and Culturing DRG Neurons – Part 1
7:29
Dissociation, Enriching, and Culturing DRG Neurons – Part 2
9:09
Results: Isolation and Culture of Dissociated Sensory Neurons from Chick Embryos
10:12
Conclusion
細胞培養モデルは、環境条件を詳細に制御を提供し、したがって、神経細胞生物学の多くの側面を解明するための強力なプラットフォームを提供します。私たちは、隔離解離、およびニワトリ胚の培養感覚ニューロンために、迅速な安価で、かつ信頼性の高い方法を記載する。基質の調製および免疫細胞化学の詳細も提供される。
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