Name: dissection of drosophila ovaries
Uploaded: 2006-10-19T00:00:00
Description: Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

<p class="jove_title"> Drosophila의 난소의 해부를 위해 프로토콜 </p><ol><li> 피드는 난소를 살찌울 그들을 해부하기 전에 효모 1-2일 함께 날아. </li><li> 마취시키다 이산화탄소 스트림을 사용하여 날아. </li><li> 족집게 한 켤레를 사용하여 여성 플라이를 선택합니다. </li><li> 1X PBS로 여성 날아 잠수함. </li><li> 핀셋 한 쌍의와의 낮은 흉부에 파리를 잡아. </li><li> 복부의 내부 장기가 노출되기 전까지는 핀셋 또 한 쌍의으로 낮은...

<ol>
	<li>Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+fragile+X+protein+functions+as+a+negative+regulator+in+the+orb+autoregulatory+pathway.">The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.</a> <em style='font-style: italic'>Dev Cell</em>. <b>8</b>, 331-342 (2005).</li><li>Tan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+autoregulatory+feedback+loop+directs+the+localized+expression+of+the+Drosophila+CPEB+protein+Orb+in+the+developing+oocyte.">An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.</a> <em style='font-style: italic'>Development</em>. <b>128</b>, 1159-1169 (2001).</li></ol>

<p> 여기 Drosophila의 난소를 해부하는 방법을 보여줍니다. culturing Drosophila의 난소에 존재하는 다른 세포 유형의 메서드가 아직 정의되었습니다로서, 이것은 Drosophila의 난소 조직을 얻기 위해 빠른 방법입니다. 기본적으로, 우리는 해부 Drosophila 난소는 간단한 2 ~ 3 단계 절차에 관련되어있다는 것을 보여줍니다. 이러한 해부하는 난소의 후속 치료를 수행하게 될 스트림 실험에 따라 달라집니다. </p>

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<td>Glass dissecting dish</td>
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dissection of drosophila ovaries

Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

Dissection of Drosophila Ovaries

Drosophila 난소의 해부

Research

JoVE Journal

Biology

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The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

연구

생물학

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6.5 DPC 마우스 태아의 해부

Analysis of gene expression patterns during early stages of mammalian embryonic development can provide important clues about gene function, cell-cell ...

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Drosophila 애벌레 NMJ의 해부

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

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Yeast is a highly tractable model system that is used to study many different cellular processes. The common laboratory strain Saccharomyces cerevisiae ...

Live Dissection of <em>Drosophila</em> Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining

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Drosophila embryos between stages 14 and 17 of embryonic development can be readily dissected to generate "fillet" preparations.  In these preparations, ...

Dissection of Oenocytes from Adult <em>Drosophila melanogaster</em>

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성인에서 Oenocytes의 해부<em> Drosophila melanogaster</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Dissection and Staining of <em>Drosophila</em> Larval Ovaries

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해부 그리고 스테 이닝<em> Drosophila</em> 애벌레 난소

Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells ...

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성인 Zebrafish의 신장의 해부

Researchers working in the burgeoning field of adult stem cell biology seek to understand the signals that regulate the behavior and function of stem ...

Dissection and Immunostaining of Imaginal Discs from <em>Drosophila melanogaster</em>

Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

해부하고, 상상 디스크의 면역 염색에서<em&gt; 초파리</em

A significant portion of post-embryonic development in the fruit fly, Drosophila melanogaster, takes place within a set of sac-like structures called ...