Name: dissection of drosophila ovaries
Uploaded: 2006-10-19T00:00:00
Description: Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

<p class="jove_title"> Protocolo para la disección de ovario de Drosophila </p><ol><li> Alimentar a las moscas con la levadura 1-2 días antes de su disección para engordar los ovarios. </li><li> Anestesiar a las moscas con flujo de dióxido de carbono. </li><li> Con un par de pinzas, seleccione una mosca hembra. </li><li> Sumerja la mosca hembra en 1X PBS. </li><li> Coge al vuelo en el tórax inferior, con un par de pinzas. </li><li> Tire suavemente en la parte inferior del abdomen con otro par de pinzas hasta que los órganos internos del a...

<ol>
	<li>Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+fragile+X+protein+functions+as+a+negative+regulator+in+the+orb+autoregulatory+pathway.">The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.</a> <em style='font-style: italic'>Dev Cell</em>. <b>8</b>, 331-342 (2005).</li><li>Tan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+autoregulatory+feedback+loop+directs+the+localized+expression+of+the+Drosophila+CPEB+protein+Orb+in+the+developing+oocyte.">An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.</a> <em style='font-style: italic'>Development</em>. <b>128</b>, 1159-1169 (2001).</li></ol>

<p> Aquí se demuestra un método de disección de los ovarios en la Drosophila. A medida que el método de cultivo de los diferentes tipos de células que existen en el ovario de Drosophila se ha definido todavía, este es un método rápido para obtener el tejido del ovario de Drosophila. En esencia, nos muestran que la disección de Drosophila ovarios implica un sencillo de dos o tres pasos. El tratamiento posterior de los ovarios disecados dependen de los experimentos posteriores que se realizarán. </p>

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<td>Forceps</td>
<td>Tool</td>
<td>Sigma</td>
<td>T43537</td>
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<td>Glass dissecting dish</td>
<td>Tool</td>
<td>Pyrex</td>
<td> 7220-85</td>
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dissection of drosophila ovaries

Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

Dissection of Drosophila Ovaries

La disección de los ovarios Drosophila

Research

JoVE Journal

Biology

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Investigación

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