Name: dissection of drosophila ovaries
Uploaded: 2006-10-19T00:00:00
Description: Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

<p class="jove_title"> Drosophila yumurtalık diseksiyon için Protokol </p><ol><li> Besleme yumurtalıkların kadar şişmanlatıyor diseksiyon maya 1-2 gün önce uçar. </li><li> Anesteziyoloji karbondioksit akışı kullanarak uçar. </li><li> Bir cımbız kullanarak, bir dişi sinek seçin. </li><li> 1X PBS içine dişi sinek Batmak. </li><li> Bir cımbız ile alt toraks sinek tut. </li><li> Başka bir çift cımbız ile karnın alt karın bölgesinde, iç organları maruz kadar hafifçe Tug. </li><li> Yumurtalıkların çifti için bakın...

<ol>
	<li>Costa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+fragile+X+protein+functions+as+a+negative+regulator+in+the+orb+autoregulatory+pathway.">The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway.</a> <em style='font-style: italic'>Dev Cell</em>. <b>8</b>, 331-342 (2005).</li><li>Tan, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+autoregulatory+feedback+loop+directs+the+localized+expression+of+the+Drosophila+CPEB+protein+Orb+in+the+developing+oocyte.">An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte.</a> <em style='font-style: italic'>Development</em>. <b>128</b>, 1159-1169 (2001).</li></ol>

<p> Burada Drosophilia&#39;daki yumurtalıklar diseksiyon yöntemi göstermektedir. Kültür Drosophila yumurtalık içinde var olan farklı hücre tipleri yöntemi henüz tanımlanmamıştır olduğu gibi, bu Drosophila yumurtalık dokusu elde etmek için hızlı bir yöntemdir. Esasen, biz diseksiyon Drosophila yumurtalıklar basit bir iki veya üç aşamalı bir prosedür içerdiğini göstermektedir. Bu disseke yumurtalıkların sonraki tedavi yapılacaktır aşağı deneylere bağlıdır. </p>

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<td>Forceps</td>
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<td>Glass dissecting dish</td>
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dissection of drosophila ovaries

Watch this Scientific Journal Video about Dissection of Drosophila Ovaries at JoVE.com

Dissection of Drosophila Ovaries

Drosophila Yumurtalıkların diseksiyonu

Research

JoVE Journal

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