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DOI :
10.3791/52014-v
November 22nd, 2014
Chapters
0:05
Title
2:26
One-step DNA Labeling Using Aziridine Cofactors (SMIIing)
5:07
Two-step Protein Labeling Using Double-activated Cofactors (mTAG)
8:41
Results: Gel Analyses for SMILing and mTAG Reactions
11:04
Conclusion
DNA及びタンパク質は、配列特異的親和性またはDNAまたはタンパク質トランスフェラーゼおよび合成補因子類似体を用いた蛍光レポーター基で標識される。酵素の補因子特異性に応じて、アジリジンまたは二活性化補助因子アナログは、一次元または二段階標識のために用いられる。
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