Protein Parçalanabilirliklerinin Sistematik Analizi muhabiri tabanlı Büyüme Deneyi

Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.