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DOI :
10.3791/52031-v
•
12:42 min
December 28th, 2014
Chapters
0:05
Title
2:20
Common Carotid Artery and Distal Middle Cerebral Artery Occlusion by Ligation
3:56
Perfusion and Sectioning of Brain Tissue
5:36
Stereological Quantification of Infiltrated Neutrophils After Cerebral Ischemia by Optical Fractionation
7:23
Brain Dissociation and Single Cell Suspension Preparation
9:41
Results: Representative Flow Cytometric Analysis of Brain Leukocytes Obtained from Naïve, Sham and Ischemic Mice
11:44
Conclusion
脳卒中後の脳骨髄細胞の特徴づけは、光分留法を用いて、立体解析によって行う、または脳白血球懸濁液のフローサイトメトリー分析により得ることができる。両方は、虚血性脳に見出さ主骨髄細胞サブセットの正確な表現型の区別を行うために有用な技術である。
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