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DOI :
10.3791/52042-v
January 12th, 2015
Chapters
0:05
Title
1:21
Poly-lysine Coated Dishes
2:04
Agarose Coated Dishes
2:38
Injection Mix and Loading Injection Needles
3:40
Sample Collection
4:38
Oocyte Injection
6:39
Fertilization, Selection, and Embryo Culture
7:55
Results: Expression of Fluorescent Proteins After mRNA Injection
8:56
Conclusion
私たちは、Branchiostomaのlanceolatumの未受精卵母細胞へのmRNA注入後、ここで蛍光タンパク質の堅牢かつ効率的な発現を報告している。この基底脊索動物におけるマイクロインジェクション技術の開発は、in vivoイメージングと遺伝子特異的な操作に含めて、この新たなモデル系で遠大な技術革新のための道を開く。
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